The use of microgel iron oxide nanoparticles in studies of magnetic resonance relaxation and endothelial progenitor cell labelling
The use of microgel iron oxide nanoparticles in studies of magnetic resonance relaxation and endothelial progenitor cell labelling
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DOI：
10.1016/j.biomaterials.2010.01.037
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发表时间：
2010-04-01
期刊：
影响因子：
14
通讯作者：
Wang, Shih-Chang
中科院分区：
文献类型：
--
作者：
Lee, Eddy S. M.;Shuter, Borys;Wang, Shih-Chang
移植后对干细胞的体内跟踪对于了解细胞命运和治疗效果至关重要。通过用磁性颗粒标记干细胞，可以通过磁共振成像(MRI)对其进行追踪。我们先前证明，微凝胶氧化铁纳米颗粒(MGIO)比商业上可获得的颗粒具有更高的跟踪灵敏度。在这里，我们描述了氧化镁的合成，并报道了其形貌、流体力学直径(87-766 nm)、氧化铁含量(高达82%)和磁化特性(M-S=52.9am-2/kg，M-R=0.061 am-2/kg和H-c=0.672 A/m)。它们的MR弛豫特性与理论模型相当，代表了模型与不同直径的实际粒子之间的首次关联。对原代内皮祖细胞的标记研究也证实，无论细胞类型如何，MGIO都是一种有效的标记。MGIO的简便合成使其成为研究磁性粒子弛豫和核磁共振细胞示踪的有用工具。(C)2010爱思唯尔有限公司。保留所有权利。
In vivo tracking of stem cells after transplantation is crucial for understanding cell-fate and therapeutic efficacy. By labelling stem cells with magnetic particles, they can be tracked by Magnetic Resonance Imaging (MRI). We previously demonstrated that microgel iron oxide nanoparticle (MGIO) provide superior tracking sensitivity over commercially available particles. Here, we describe the synthesis of MGIO and report on their morphology, hydrodynamic diameters (87-766 nm), iron oxide weight content (up to 82%) and magnetization characteristics (M-s = 52.9 Am-2/kg, M-R = 0.061 Am-2/kg and H-c = 0.672 A/m). Their MR relaxation characteristics are comparable to those of theoretical models and represent the first such correlation between model and real particles of varying diameters. A labelling study of primary endothelial progenitor cells also confirms that MGIO is an efficient label regardless of cell type. The facile synthesis of MGIO makes it a useful tool for the studying of relaxation induced by magnetic particles and cellular tracking by MRI. (C) 2010 Elsevier Ltd. All rights reserved.