Ribosomal protein S1 is not essential for the trans-translation machinery
Ribosomal protein S1 is not essential for the trans-translation machinery
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DOI：
10.1016/j.jmb.2007.02.068
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发表时间：
2007-05-04
影响因子：
5.6
通讯作者：
Ueda, Takuya
中科院分区：
文献类型：
--
作者：
Qi, Hao;Shimizu, Yoshihiro;Ueda, Takuya
在真细菌中，核糖体在蛋白质合成过程中被tmrna衍生的反翻译系统拯救。由于核糖体蛋白S1以高亲和力特异性结合tmRNA，因此被认为参与了反翻译系统。然而，S1在翻译中的作用尚不清楚。为了研究S1在翻译系统中的作用，我们构建了一个无S1的无细胞翻译系统。我们发现，即使在没有S1的情况下，反译仍在进行。在无S1体系中加入S1并不影响翻译效率。这些结果表明S1在翻译机制中不起作用。(c) 2007 Elsevier Ltd.版权所有。
In eubacteria, ribosome stalling during protein synthesis is rescued by a tmRNA-derived trans-translation system. Because ribosomal protein S1 specifically binds to tmRNA with high affinity, it is considered to be involved in the trans-translation system. However, the role of S1 in trans-translation is still unclear. To study the function of S1 in the trans-translation system, we constructed an S1-free cell-free translation system. We found that trans-translation proceeded even in the absence of S1. Addition of S1 into the S1-free system did not affect trans-translation efficiency. These results suggest that S1 does not play a role in the trans-translation machinery. (c) 2007 Elsevier Ltd. All rights reserved.