Expression Analyses of Vitellogenin and Target of Rapamycin of Sogatella furcifera (Hemiptera: Delphacidae), and Their Effects on Reproduction
Expression Analyses of Vitellogenin and Target of Rapamycin of Sogatella furcifera (Hemiptera: Delphacidae), and Their Effects on Reproduction
复制标题
DOI：
10.1093/jee/toab195
复制
发表时间：
2021-10
影响因子：
2.2
通讯作者：
Jinyu Yi;Fei Li;Chang Xu;Yudi Liu;M. Hou
中科院分区：
文献类型：
--
作者：
Jinyu Yi;Fei Li;Chang Xu;Yudi Liu;M. Hou
摘要 卵黄原蛋白（Vg）和雷帕霉素靶标（TOR）是参与昆虫繁殖调控的重要基因。本工作克隆了白背飞虱的 Vg (SfVg) 和 TOR (SfTOR) 基因全长。女性中的表达模式显示，SfVg在脂肪体中高表达，SfTOR在马氏小管中高表达。沉默 SfVg 或 SfTOR 后，雌性成虫不再产卵。他们的卵巢发育延迟，卵母细胞中的卵黄蛋白沉积减少。然而，野生型雌性与 SfTOR 沉默的雄性交配可以正常产卵并产生后代。对 SfTOR 敲低的雄性睾丸和附属腺的解剖表明，它们的发育没有受到影响。因此，沉默SfVg或SfTOR基因可以有效抑制雌性生殖，但敲低SfTOR对雄性生殖能力没有显着影响。此外，沉默 SfTOR 会导致 SfVg 表达显着降低。上述结果表明，SfVg和SfTOR对于白背飞虱的繁殖至关重要，并可能为害虫防治提供潜在的靶标。
Abstract Vitellogenin (Vg) and the target of rapamycin (TOR) are important genes involved in insect reproduction regulation. In this work, the full lengths of the Vg (SfVg) and TOR (SfTOR) genes of the white-backed planthopper Sogatella furcifera were cloned. The expression pattern in females showed that SfVg was highly expressed in fat bodies, and SfTOR was highly expressed in Malpighian tubules. After silencing SfVg or SfTOR, female adults did not deposit eggs. Their ovarian development was delayed, and yolk protein deposition in the oocytes was reduced. However, wild-type females mated with SfTOR-silenced males could lay eggs and produce offsprings normally. The dissections of testes and accessory glands of males with SfTOR knockdown showed that their development was not affected. Therefore, the silencing of the SfVg or SfTOR genes can effectively inhibit female reproduction, but SfTOR knockdown has no significant effect on male reproductive capacity. Furthermore, silencing SfTOR can cause SfVg expression to decrease significantly. All of the above results revealed that SfVg and SfTOR are essential for white-backed planthopper reproduction and may provide a potential target for pest control.