Comparison of Different Solvents and Extraction Methods for Isolation of Phenolic Compounds from Horseradish Roots (Armoracia rusticana)
Comparison of Different Solvents and Extraction Methods for Isolation of Phenolic Compounds from Horseradish Roots (Armoracia rusticana)
复制标题
DOI：
--
复制
发表时间：
2012-04
期刊：
影响因子：
--
通讯作者：
L. Tomsone;Z. Kruma;R. Galoburda
中科院分区：
文献类型：
--
作者：
L. Tomsone;Z. Kruma;R. Galoburda
-辣根(Aroracia Rusticana)是十字花科多年生草本植物，含有生物活性物质。本研究的目的是确定从辣根中提取具有较高抗自由基活性的酚类化合物的最佳方法。用正己烷、乙酸乙酯、二乙醚、异丙醇、丙酮、乙醇(95%)、乙醇/水/冰醋酸(80/20/1v/v/v/v)和乙醇/水(体积比80/20)两种提取方法，用8种不同的溶剂(正己烷、乙酸乙酯、二乙醚、异丙醇、丙酮)和乙醇/水(体积比为80/20)提取辣根的3种基因型(编号105、编号106和品种‘图尔库’)。乙醇和乙醇/水作为最佳溶剂可供选择。虽然索氏提取物的TPC较高，但对DPPH·自由基的清除能力并未增强。由此可见，采用索氏提取法提取的化合物较多，不是有效的抗氧化剂。
— Horseradish ( Armoracia rusticana ) is a perennial herb belonging to the Brassicaceae family and contains biologically active substances. The aim of the current research was to determine best method for extraction of phenolic compounds from horseradish roots showing high antiradical activity. Three genotypes (No. 105; No. 106 and variety ‘Turku’) of horseradish roots were extracted with eight different solvents: n-hexane, ethyl acetate, diethyl ether, 2-propanol, acetone, ethanol (95%), ethanol / water / acetic acid (80/20/1 v/v/v) and ethanol / water (80/20 by volume) using two extraction methods (conventional and Soxhlet). As the best solvents ethanol and ethanol / water solutions can be chosen. Although in Soxhlet extracts TPC was higher, scavenging activity of DPPH˙ radicals did not increase. It can be concluded that using Soxhlet extraction method more compounds that are not effective antioxidants.