Three Inhibitors of FGF Receptor, ERK, and GSK3 Establishes Germline-Competent Embryonic Stem Cells of C57BL/6N Mouse Strain With High Efficiency and Stability
Three Inhibitors of FGF Receptor, ERK, and GSK3 Establishes Germline-Competent Embryonic Stem Cells of C57BL/6N Mouse Strain With High Efficiency and Stability
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DOI：
10.1002/dvg.20614
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发表时间：
2010-05-01
期刊：
影响因子：
1.5
通讯作者：
Aizawa, Shinichi
中科院分区：
文献类型：
--
作者：
Kiyonari, Hiroshi;Kaneko, Mari;Aizawa, Shinichi
C57BL/6小鼠是小鼠遗传学中最标准的品系。然而，这种菌株确实有几个缺点；其中之一是建立胚胎干细胞（ES）的困难。目前还没有可靠的C57BL/6 ES细胞系广泛用于基因靶向构建突变小鼠。如果人们能够通过稳定培养生殖系胜任的C57BL/6胚胎干细胞常规地产生多种突变，或者如果人们能够从C57BL/6衍生的突变小鼠中常规地建立胚胎干细胞以产生多种突变，这也极大地有利于小鼠遗传学。最近，一种含有三种抑制剂（3i: SU5402用于FGFR， PD184352用于ERK， CHIR99021用于G5K3）的ES培养方法被报道。在这里，我们证明了这种3i方法在C57BL/6N菌株中建立和培养生殖系胚胎干细胞是非常有用的。创世纪48:317 - 327,2010。(C) 2010 Wiley-Liss, Inc。
C57BL/6 mouse is the most standard strain in mouse genetics. The strain does, however, have several disadvantages; one being the difficulty in establishing embryonic stem (ES) cells. No reliable C57BL/6 ES cell line is widely available for creating mutant mice through gene targeting. It also greatly favors mouse genetics if one can routinely make multiple mutations by stably culturing germline-competent C57BL/6 ES cells or if one can routinely establish ES cells from C57BL/6-derived mutant mice to make multiple mutations. Recently, an ES culture method with three inhibitors (3i: SU5402 for FGFR, PD184352 for ERK, and CHIR99021 for G5K3) has been reported. Here we show that this 3i method is extremely instrumental in establishing and culturing germline-competent ES cells in the C57BL/6N strain. genesis 48:317-327,2010. (C) 2010 Wiley-Liss, Inc.