Spermidine Enhances Waterlogging Tolerance via Regulation of Antioxidant Defence, Heat Shock Protein Expression and Plasma Membrane H+-ATPase Activity in Zea mays
Spermidine Enhances Waterlogging Tolerance via Regulation of Antioxidant Defence, Heat Shock Protein Expression and Plasma Membrane H+-ATPase Activity in Zea mays
复制标题
DOI：
10.1111/jac.12058
复制
发表时间：
2014-06-01
影响因子：
3.5
通讯作者：
Zhang, J.
中科院分区：
文献类型：
--
作者：
Liu, M. Y.;Sun, J.;Zhang, J.
多胺（Polyamines，PAs）是一类普遍存在的生物胺，在作物的抗逆过程中起着重要作用。然而，多胺与植物耐涝性的关系却很少受到关注。以玉米为材料，研究了亚精胺（Spd）对WL胁迫的保护作用。WL处理抑制了玉米根系的生长，并导致玉米根系膜脂过氧化、细胞膜透性和DNA损伤显著增加。Spd浸种可明显减轻WL对种子的伤害。Spd预浸处理也显著提高了对照和淹水玉米根系内源Spd含量。此外，WL胁迫下，Spd预处理的玉米根系抗氧化酶（CAT、GR、SOD、POD）活性增强。因此，过氧化氢（H2 O2）和超氧自由基（O2中心点-）的积累减少。Spd处理还增强了WL胁迫期间热休克蛋白（HSP）70和90的表达和蛋白丰度。此外，Spd预处理可逆转WL对玉米根系H+流出和K+流入的抑制作用。在WL条件下，Spd预处理对质膜H+-ATPase活性的抑制作用较小。总之，本研究表明，WL胁迫可以缓解引发种子与亚精胺在玉米物种。这种抑制作用部分归因于（i）上调抗氧化酶的活性;（ii）维持PM H+-ATP酶活性;和（iii）增加Hsp 70和Hsp 90基因表达和蛋白质丰度。
Polyamines (PAs) are ubiquitous biogenic amines that play important roles in the environmental stress tolerance of crops. However, the relationship between PAs and plant waterlogging (WL) tolerance has received little attention. In this study, the protective effects of spermidine (Spd) against WL stress were investigated by Zea mays. WL treatment inhibited root growth and caused a significant increase in lipid peroxidation, membrane permeability and DNA damage in maize roots. Pre-soaking seeds with Spd markedly alleviated these effects caused by WL. Spd pre-soaking also significantly increased the endogenous Spd content in both control and waterlogged maize roots. In addition, the activity of antioxidant enzymes (catalase, CAT; glutathione reductase, GR; superoxide dismutase, SOD; peroxidase, POD) was enhanced in Spd-pre-soaked maize roots under WL stress. Thus, hydrogen peroxide (H2O2) and superoxide-radical (O2 center dot-) accumulation were reduced. Spd treatment also enhances the expression and protein abundance of heat shock protein (HSP) 70 and 90 during the period of WL stress. Furthermore, Spd pre-soaking reversed the inhibition effect of WL on H+ efflux and K+ influx in maize roots. The PM H+-ATPase activity in Spd-pre-treated seedlings exhibited less inhibitory under WL conditions. In conclusion, this study suggests that WL stress could be alleviated by priming seeds with Spd in maize species. This alleviative effect was partially attributable to (i) up-regulated activity of antioxidant enzymes; (ii) maintained PM H+-ATPase activity; and (iii) increased Hsp70 and Hsp90 gene expression and protein abundance.