Dietary and plasma carotenoids are positively associated with alpha diversity in the fecal microbiota of pregnant women.
Dietary and plasma carotenoids are positively associated with alpha diversity in the fecal microbiota of pregnant women.
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DOI：
10.1111/1750-3841.15586
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发表时间：
2021-02
影响因子：
3.9
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中科院分区：
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由于微生物使用类胡萝卜素作为抗氧化剂进行保护，因此膳食类胡萝卜素可能与肠道微生物群组成有关。我们的目的是确定孕妇类胡萝卜素摄入量、血浆类胡萝卜素浓度和粪便细菌群落之间的关系。孕妇（n = 27）入组了一项2组研究，旨在评估生物标本采集和实际营养干预的可行性。收集血浆和粪便样本，并对女性进行24小时饮食清单和回忆调查。血浆类胡萝卜素通过使用光电二极管阵列检测的HPLC分析。通过16S rRNA DNA测序分析粪便细菌。由于干预组和常规护理组之间缺乏显著差异（n = 23例数据完整的女性），因此所列结果是两个研究组合并的36周妊娠研究访视的横断面结果。最近摄入的含类胡萝卜素的食物包括胡萝卜，甘薯，芒果，甜菜，和/或甜椒（48%）;橙子/橙子汁（17%）;鸡蛋（39%）;番茄/番茄酱（52%）;水果（83%）;蔬菜（65%）。平均血浆类胡萝卜素浓度为6.4ug/dL α-胡萝卜素（AC）、17.7ug/dL β-胡萝卜素（BC）、11.4ug/dL隐黄质（CR）、39.0ug/dL反式番茄红素（TL）和29.8ug/dL玉米黄质和叶黄素（ZL）。AC和BC浓度较高的妇女谁最近消费的食物高类胡萝卜素。在食用橙子/橙子汁的女性中，CR浓度较高。微生物α多样性与AC和BC呈正相关。微生物群β多样性在所报告的含类胡萝卜素食物的摄入量和AC的血浆浓度之间存在显著差异。这可能反映了高纤维或改善整体饮食质量的效果，而不是类胡萝卜素的特定效果。
Since microbes use carotenoids as an antioxidant for protection, dietary carotenoids could be associated with gut microbiota composition. We aimed to determine associations among reported carotenoid intake, plasma carotenoid concentrations, and fecal bacterial communities in pregnant women. Pregnant women (n=27) were enrolled in a 2-arm study designed to assess feasibility of biospecimen collection and delivery of a practical nutrition intervention. Plasma and fecal samples were collected and women were surveyed with a 24-hour dietary checklist and recalls. Plasma carotenoids were analyzed by HPLC using photodiode array detection. Fecal bacteria were analyzed by 16S rRNA DNA sequencing. Results presented are cross-sectional from the 36-week gestational study visit combined across both study arms due to lack of significant differences between intervention and usual care groups (n=23 women with complete data). Recent intake of carotenoid-containing foods included carrots, sweet potatoes, mangos, apricots, and/or bell peppers for 48% of women; oranges/orange juice (17%); egg (39%); tomato/tomato-based sauces (52%); fruits (83%); vegetables (65%). Average plasma carotenoid concentrations were 6.4ug/dL α-carotene (AC), 17.7ug/dL β-carotene (BC), 11.4ug/dL cryptoxanthin (CR), 39.0ug/dL trans-lycopene (TL), and 29.8ug/dL zeaxanthin and lutein (ZL). AC and BC concentrations were higher in women who recently consumed foods high in carotenoids. CR concentrations were higher in women who consumed oranges/orange juice. Microbiota α-diversity positively correlated with AC and BC. Microbiota β-diversity differed significantly across reported intake of carotenoid containing foods and plasma concentrations of AC. This may reflect an effect of high fiber or improved overall dietary quality, rather than a specific effect of carotenoids.