Effects of Long-Term Water-Aging on Novel Anti-Biofilm and Protein-Repellent Dental Composite.
Effects of Long-Term Water-Aging on Novel Anti-Biofilm and Protein-Repellent Dental Composite.
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长期水老化对新型抗生物膜和蛋白质排斥性牙科复合材料的影响
DOI：
10.3390/ijms18010186
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发表时间：
2017-01-18
影响因子：
5.6
通讯作者：
Xu HH
中科院分区：
文献类型：
--
作者：
Zhang N;Zhang K;Melo MA;Weir MD;Xu DJ;Bai Y;Xu HH
本研究的目的是：（1）通过将2-甲基丙烯酰氧乙基磷酰胆碱（MPC）与甲基丙烯酸二甲氨基十六烷基酯季铵盐（DMAHDM）结合，合成一种抗生物膜和蛋白质排斥的牙科复合材料;（2）评价水老化180 d对MPC-DMAHDM复合材料的蛋白质抵抗性、杀菌能力和机械性能的影响。将MPC和DMAHDM加入到树脂复合物中。将样本在37 °C的蒸馏水中储存1、30、90和180天。在三点弯曲中测量机械性能。蛋白质附着到复合材料上进行了评估，通过微bicinchoninic酸的方法。采用口腔菌斑微宇宙生物膜模型来评估口腔生物膜活力与水老化时间。浸泡180天后，MPC-DMAHDM复合材料的机械性能与商业对照复合材料的机械性能相匹配。含3%MPC +1.5%DMAHDM的复合材料的抗蛋白粘附能力明显强于对照组（p < 0.05）。MPC + DMAHDM比单独MPC或DMAHDM获得更强的生物膜根除效果（p < 0.05）。3%MPC +1.5%DMAHDM复合材料上的生物膜菌落形成单位比商业对照低三个数量级。蛋白质驱避和抗菌效果持久，在1至180天的水老化中没有损失。新型MPC-DMAHDM复合材料具有强大而持久的抗蛋白质粘附和有效的细菌清除功能，同时与商业牙科复合材料的承载能力相匹配。新型MPC-DMAHDM复合物代表了抑制口腔菌斑生长、酸产生和继发性龋齿的有前景的手段。
The aims of this study were to: (1) synthesize an anti-biofilm and protein-repellent dental composite by combining 2-methacryloyloxyethyl phosphorylcholine (MPC) with quaternary ammonium dimethylaminohexadecyl methacrylate (DMAHDM); and (2) evaluate the effects of water-aging for 180 days on protein resistance, bacteria-killing ability, and mechanical properties of MPC-DMAHDM composite. MPC and DMAHDM were added into a resin composite. Specimens were stored in distilled water at 37 °C for 1, 30, 90, and 180 days. Mechanical properties were measured in three-point flexure. Protein attachment onto the composite was evaluated by a micro bicinchoninic acid approach. An oral plaque microcosm biofilm model was employed to evaluate oral biofilm viability vs. water-aging time. Mechanical properties of the MPC-DMAHDM composite after 180-day immersion matched those of the commercial control composite. The composite with 3% MPC + 1.5% DMAHDM had much stronger resistance to protein adhesion than control (p < 0.05). MPC + DMAHDM achieved much stronger biofilm-eradicating effects than MPC or DMAHDM alone (p < 0.05). Biofilm colony-forming units on the 3% MPC + 1.5% DMAHDM composite were three orders of magnitude lower than commercial control. The protein-repellent and antibacterial effects were durable and showed no loss in water-aging from 1 to 180 days. The novel MPC-DMAHDM composite possessed strong and durable resistance to protein adhesion and potent bacteria-eradicating function, while matching the load-bearing ability of a commercial dental composite. The novel MPC-DMAHDM composite represents a promising means of suppressing oral plaque growth, acid production, and secondary caries.