The Role of Low-Molecular-Weight Organic Carbons in Facilitating the Mobilization and Biotransformation of As(V)/Fe(III) from a Realgar Tailing Mine Soil
The Role of Low-Molecular-Weight Organic Carbons in Facilitating the Mobilization and Biotransformation of As(V)/Fe(III) from a Realgar Tailing Mine Soil
复制标题
低分子量有机碳在促进雄黄尾矿矿土壤中 As(V)/Fe(III) 的移动和生物转化中的作用
DOI：
10.1080/01490451.2018.1429506
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发表时间：
2018-01-01
影响因子：
2.3
通讯作者：
Wang, Yuanpeng
中科院分区：
文献类型：
--
作者：
Chen, Zheng;Dong, Guowen;Wang, Yuanpeng
将几种低分子量有机碳（LMWOC）化合物（乙酸盐、丙酸盐、丁酸盐、乳酸盐和葡萄糖）添加到富砷尾矿土壤中，研究它们对富砷尾矿土壤中As/Fe的活化和微生物群落的潜在迁移的影响。与单独使用去离子水的生物微宇宙相比，补充LMWOCs基质对土壤中As（V）/Fe（III）的动员和生物转化具有促进作用。在38 d的培养过程中，添加低分子有机碳基质的土壤中As（III）和Fe（II）的释放量分别超过2100 g/L和4.2mg/L，而单独添加去离子水的土壤中As（III）和Fe（II）的释放量分别低于35 g/L和1.82mg/L。PCR-DGGE结果表明，几种低分子量有机碳反应的细菌主要与厚壁菌门和变形菌门有关。此外，一个可以忽略不计的影响，对丰富的Fe（III）-还原家庭Geobacteraceae表明在LMWOCs修正土壤。然而，硫酸盐还原菌的丰度增加，但砷酸盐呼吸细菌的丰度减少，表明在土壤中单独补充乙酸盐，与其他低分子量有机碳修正案相比。DNA稳定同位素探针分析表明，乙酸盐在土壤中不仅作为As（V）/Fe（III）生物转化的电子供体，而且作为一种强有力的能源被同化，促进硫酸盐还原菌的生长。研究结果表明，存在着特定的细菌，它们优先响应于LMWOC的添加来控制土壤中As/Fe的生化循环过程。
Several low-molecular-weight organic carbon (LMWOC) compounds (acetate, propionate, butyrate, lactate, and glucose) were added to flooded arsenic-rich tailing mine soil to investigate their effect to the mobilization of As/Fe and potential shift of microbial community. A promoting effect to the mobilization and biotransformation of As(V)/Fe(III) in the soils resulting from the supplementation with LMWOCs substrate was indicated compared to the biotic microcosm amended with deionized water alone. During 38-day biotic incubation, more than 2100g/L of As(III) and 4.2mg/L of Fe(II) levels were released from the soils amended with LMWOCs substrates, compared to the levels of As(III) and Fe(II) (less 35g/L and 1.82mg/L) derived from the biotic supplementation with deionized water alone. PCR-DGGE indicated that several LMWOCs-responded bacteria were mostly related to Firmicutes and Proteobacteria. Moreover, a negligible impact on the abundance of Fe(III)-reducing family Geobacteraceae was indicated in the LMWOCs-amended soils. However, an increased abundance of sulfate-reducing bacteria but a decreased abundance of arsenate-respiring bacteria were indicated upon the soils supplemented with acetate alone, compared with other LMWOC amendments. DNA-stable isotope probing analysis demonstrated that the dual roles of acetate was not only served as an electron donor for biotransformation of As(V)/Fe(III) in soil, but also assimilated as a powerful energy source to promote the growth of sulfate-reducing bacteria. The findings suggest that there are specific bacteria that preferentially respond to the additions of LMWOC for controlling the biochemical cycle process of As/Fe in soils.