The influence of different calorie restriction protocols on serum pro-inflammatory cytokines, adipokines and IGF-I levels in female C57BL6 mice: short term and long term diet effects.
The influence of different calorie restriction protocols on serum pro-inflammatory cytokines, adipokines and IGF-I levels in female C57BL6 mice: short term and long term diet effects.
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DOI：
10.1016/j.mgene.2016.12.013
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发表时间：
2017-06
期刊：
影响因子：
0.7
通讯作者：
Cleary MP
中科院分区：
文献类型：
--
作者：
Dogan S;Ray A;Cleary MP
热量限制（CR）是预防包括癌症在内的慢性疾病的有效干预措施。虽然有很多因素，例如，性激素、IGF-I和mTOR对CR的反应已被研究，CR的分子机制仍有待确定。我们的目的是确定不同CR方案对促炎细胞因子的短期和长期影响。我们的假设是，与慢性CR（CCR）相比，间歇性CR（ICR）将导致对促炎血清细胞因子的更大抑制，因为我们之前发现ICR在预防乳腺肿瘤发展方面更具保护性。从10周龄开始，雌性C57 BL 6小鼠维持在随意（AL）喂食、ICR或CCR方案（总体CR为AL的约75%）下长达74周龄。在指定的年龄段采集血样，测定血清白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、脂联素、瘦素、胰岛素样生长因子-I（IGF-I）和胰岛素。对于ICR小鼠，在限制3周（ICR-R）和再喂养1周（ICR-RF）后采集样品。总体而言，与AL相比，两种CR模式均显著降低血清IL-6、TNF-α、IGF-I和瘦素水平，在研究终止时，ICR组和CCR组的IL-6水平分别降低24倍和3.5倍，TNF-α水平分别降低11倍和1.5倍。ICR-RF小鼠中脂联素和胰岛素有最高的趋势。体重与IL-6、TNF-α、胰岛素、瘦素呈正相关，与脂联素/瘦素比值呈负相关。IL-6与TNF-α呈正相关。ICR的有益作用可能通过促炎细胞因子途径发挥作用。
Calorie restriction (CR) is an effective intervention to prevent chronic diseases including cancer. Although many factors, i.e., sex hormones, IGF-I and mTOR have been studied in response to CR, the molecular mechanisms of CR remain to be identified. Our objective was to determine the short and long-term effects of different CR protocols on pro-inflammatory cytokines. Our hypothesis was that Intermittent CR (ICR) would result in greater inhibition of pro-inflammatory serum cytokines compared to Chronic CR (CCR) as we previously found ICR to be more protective in the prevention of mammary tumor development. From ten weeks of age female C57BL6 mice were maintained on either ad libitum (AL) fed, ICR or CCR protocols (overall CR of ~75% of AL) for up to 74 weeks of age. Blood samples were collected for measurements of serum interleukin-6 (IL-6), tumor necrosis factor–alpha (TNF-α), adiponectin, leptin, IGF-I and insulin at specified ages. For ICR mice samples were collected following 3 weeks of restriction (ICR-R) and after one week of refeeding (ICR-RF). In general, both modes of CR significantly reduced serum IL-6, TNF-α, IGF-I and leptin levels compared to AL with IL-6 levels 24 and 3.5 fold and TNF-α levels t 11 and 1.5 fold lower in ICR and CCR groups, respectively at study termination. There was a trend for adiponectin and insulin to be highest in ICR-RF mice. Body weights were positively correlated with IL-6, TNF-α, insulin and leptin but negatively correlated with adiponectin-to-leptin ratio. Moreover, there was a positive correlation between IL-6 and TNF-α. Beneficial effects of ICR may function through pro-inflammatory cytokine pathways.