The RNA Helicase eIF4A Is Required for Sapovirus Translation.
The RNA Helicase eIF4A Is Required for Sapovirus Translation.
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DOI：
10.1128/jvi.03174-15
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发表时间：
2016-05-15
影响因子：
5.4
通讯作者：
Cho KO
中科院分区：
文献类型：
--
作者：
Hosmillo M;Sweeney TR;Chaudhry Y;Leen E;Curry S;Goodfellow I;Cho KO
真核生物起始因子4A（eIF4A）是一种DEAD盒解旋酶，可解旋mRNA 5′非翻译区（UTR）的RNA结构。在这里，我们研究了eIF4A在猪痘病毒VPg依赖性翻译中的作用。使用抑制剂和显性阴性突变体，我们发现eIF4A是病毒翻译和感染性所必需的，这表明尽管存在非常短的5′ UTR，但eIF4A是解链sapovirus基因组中RNA结构以促进病毒翻译所必需的。
The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5′ untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5′ UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.