A novel in situ urinary bladder model for studying afferent and efferent mechanisms in the micturition reflex in the rat
A novel in situ urinary bladder model for studying afferent and efferent mechanisms in the micturition reflex in the rat
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DOI：
10.1002/nau.22435
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发表时间：
2014-06-01
影响因子：
2
通讯作者：
Tobin, Gunnar
中科院分区：
文献类型：
--
作者：
Aronsson, Patrik;Carlsson, Thomas;Tobin, Gunnar
目的寻找新的动物模型来研究排尿反射的传出和传入水平，对更好地理解排尿功能障碍具有重要意义。因此，在本研究中，我们开发了一种新的原位膀胱模型，并与传统的全膀胱模型进行了比较。方法用戊巴比妥和美托咪定麻醉的雄性大鼠，精心制备膀胱，经中线切口分离膀胱至尿道。分离的两半膀胱（没有直接连接）用结扎固定在底层组织上。张力可以在一侧被记录下来，而另一半偶尔被拉伸，以唤起传入信号。同时采用ATP、甲胆碱注射和神经电刺激。结果30和50mN的同侧拉伸诱导了对侧的力依赖性收缩反应。此外，电刺激盆腔传出神经纤维，静脉注射甲胆碱和ATP，引起剂量依赖性收缩，类似于在全膀胱模型中观察到的反应。图中，电刺激传出纤维时的阈值频率为
Aims The search for new animal models to investigate both efferent and afferent levels of the micturition reflex, to better understand urinary dysfunctions, is of great importance. Therefore in this study we developed and characterized, by comparisons with a conventional whole bladder model, a novel in situ model. Methods The urinary bladder was carefully prepared and separated, via a midline incision, into two halves all the way to the urethra in pentobarbitone and medetomidine anesthetized male rats. The separated bladder halves (with no direct connection) were immobilized with ligatures to the underlying tissue. The tension could thereafter be recorded at one side, while the other half was occasionally stretched in order to evoke an afferent signal. Also, injections of ATP and methacholine and electric nerve stimulation were employed. Results Ipsilateral stretch of 30 and 50mN induced a force-dependent contractile response on the contralateral side. Moreover, electrical stimulation of efferent pelvic nerve fibers, and intravenous injections of methacholine and ATP, evoked dose-dependent contractions, resembling responses observed in the whole bladder model. Here, the threshold frequency at electrical stimulation of the efferent fibers was