Blood, sweat, and tears: Red Blood Cell-Omics study objectives, design, and recruitment activities
Blood, sweat, and tears: Red Blood Cell-Omics study objectives, design, and recruitment activities
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DOI：
10.1111/trf.14971
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发表时间：
2019-01-01
期刊：
影响因子：
2.9
通讯作者：
Cristman, A. M.
中科院分区：
文献类型：
--
作者：
Endres-Dighe, Stacy M.;Guo, Yuelong;Cristman, A. M.
背景：红细胞 (RBC) 组学研究的目的是建立一个大型数据集，其中包含献血者的行为、遗传和生化特征，并与输注其捐赠的红细胞的患者的结果相关。 研究设计和方法：该队列是从四个美国血液中心招募的。人口统计和捐赠数据来自中心记录。入组时完成了评估异食癖、不宁腿综合征、铁补充剂、激素使用以及月经和妊娠史的调查问卷。采集血液进行全血细胞计数、DNA 和铁蛋白检测。在储存第 39 至 42 天，将白细胞减少的红细胞样本转移到定制储存袋中进行溶血测试。召回一个子集以评估溶血措施的动力学和稳定性。 结果：共有 13,403 名不同种族/民族（12% 非洲裔美国人、12% 亚洲人、8% 西班牙裔、64% 白人和 5% 多种族/其他）的男女捐献者已登记，年龄范围为 18 岁至 90 岁； 15% 是高强度捐献者（过去 24 个月内有 9 次或更多捐献，没有低血红蛋白延期）。该队列中 97% 至 99% 的数据元素可用。结论：该队列为与铁代谢、铁缺乏的不良后果以及红细胞储存期间差异溶血（包括氧化和渗透应激扰动）相关的广泛献血者研究提供了人口统计、行为、生化和遗传数据。与受者结果的联系可能有助于分析供者特征如何影响输血效果。储存库 DNA、血浆和红细胞样本应扩大当前数据集的用途。
BACKGROUND: The Red Blood Cell (RBC)-Omics study was initiated to build a large data set containing behavioral, genetic, and biochemical characteristics of blood donors with linkage to outcomes of the patients transfused with their donated RBCs.STUDY DESIGN AND METHODS: The cohort was recruited from four US blood centers. Demographic and donation data were obtained from center records. A questionnaire to assess pica, restless leg syndrome, iron supplementation, hormone use, and menstrual and pregnancy history was completed at enrollment. Blood was obtained for a complete blood count, DNA, and ferritin testing. A leukocyte-reduced RBC sample was transferred to a custom storage bag for hemolysis testing at Storage Days 39 to 42. A subset was recalled to evaluate the kinetics and stability of hemolysis measures.RESULTS: A total of 13,403 racially/ethnically diverse (12% African American, 12% Asian, 8% Hispanic, 64% white, and 5% multiracial/other) donors of both sexes were enrolled and ranged from 18 to 90 years of age; 15% were high-intensity donors (nine or more donations in the prior 24 mo without low hemoglobin deferral). Data elements are available for 97% to 99% of the cohort.CONCLUSIONS: The cohort provides demographic, behavioral, biochemical, and genetic data for a broad range of blood donor studies related to iron metabolism, adverse consequences of iron deficiency, and differential hemolysis (including oxidative and osmotic stress perturbations) during RBC storage. Linkage to recipient outcomes may permit analysis of how donor characteristics affect transfusion efficacy. Repository DNA, plasma, and RBC samples should expand the usefulness of the current data set.