SHARED ALLELIC LOSSES ON CHROMOSOMES 1P AND 19Q SUGGEST A COMMON ORIGIN OF OLIGODENDROGLIOMA AND OLIGOASTROCYTOMA
SHARED ALLELIC LOSSES ON CHROMOSOMES 1P AND 19Q SUGGEST A COMMON ORIGIN OF OLIGODENDROGLIOMA AND OLIGOASTROCYTOMA
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DOI：
10.1097/00005072-199501000-00011
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发表时间：
1995-01-01
影响因子：
3.2
通讯作者：
VONDEIMLING, A
中科院分区：
文献类型：
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作者：
KRAUS, JA;KOOPMANN, J;VONDEIMLING, A
可能含有肿瘤抑制基因的特定染色体区域的杂合性缺失(LOH)与人类胶质瘤有关。本研究分析了星形胶质细胞和少突胶质细胞肿瘤1和19号染色体上的LOH。微卫星分析发现6/15例WHO II和III级少突胶质细胞瘤，12/25例WHO II和III级少突胶质细胞瘤，6/79例WHO IV级胶质母细胞瘤，5/44例WHO II和III级星形胶质细胞瘤，0/23例毛细胞性星形细胞瘤。此外，染色体臂Ip在少突胶质细胞瘤和少突胶质细胞瘤中频繁参与，但在星形细胞瘤中不参与，这表明少突胶质细胞瘤在遗传学上更类似于少突胶质细胞瘤而不是星形细胞瘤。为了支持这一假说，对三个混合性少星形细胞瘤的少突胶质细胞和星形细胞区进行了LOH 1p和LOH 19q的不同检查，LOH 19q是据信在这些肿瘤中受影响的第二个遗传区域。三种肿瘤在少突胶质细胞和星形细胞分化区的LOH分别为1p和19q。这些发现表明，寡星形细胞瘤的星形细胞和少突胶质细胞部分具有相同的分子遗传学特征，可能是单克隆性起源。
Loss of heterozygosity (LOH) in specific chromosomal regions, which are likely to harbor tumor suppressor genes, has been associated with human gliomas. In this study we have analyzed astrocytic and oligodendroglial tumors for LOH on chromosomes 1 and 19. By microsatellite analysis LOH was found on chromosome arm 1p in 6/15 oligodendrogliomas WHO grade II and III, 12/25 oligoastrocytomas WHO grade II and III, 6/79 glioblastomas WHO grade IV, 5/44 astrocytomas WHO grade II and III and 0/23 pilocytic astrocytomas WHO grade I. The high incidence of LOH on chromosome arm 1p in oligodendrogliomas and oligoastrocytomas indicates that a putative tumor suppressor gene in this region is involved in the formation of gliomas with oligodendroglial features. Furthermore, the frequent involvement of chromosome arm Ip in oligodendrogliomas and oligoastrocytomas, but not in astrocytomas, suggests that genetically oligoastrocytoma is more similar to oligodendroglioma than to astrocytoma. In order to support this hypothesis, oligodendroglial and astrocytic areas in three mixed oligoastrocytomas were examined differentially for LOH 1p and for LOH 19q, the second genetic region believed to be affected in these tumors. All three tumors had LOH of 1p and LOH of 19q in both areas of oligodendroglial and of astrocytic differentiation. These findings show that the astrocytic and oligodendroglial portions of oligoastrocytoma share molecular genetic features and probably are of monoclonal origin.