Comparative transcriptome analyses reveal two distinct transcriptional modules associated with pollen shedding time in pine
Comparative transcriptome analyses reveal two distinct transcriptional modules associated with pollen shedding time in pine
复制标题
比较转录组分析揭示了与松树花粉脱落时间相关的两个不同的转录模块
DOI：
10.1186/s12864-020-06880-9
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发表时间：
2020-07
期刊：
影响因子：
4.4
通讯作者：
Niu Shi-Hui
中科院分区：
文献类型：
--
作者：
Ma Jing-Jing;Liu Shuang-Wei;Han Fang-Xu;Li Wei;Li Yue;Niu Shi-Hui
背景季节性开花时间是温带树木的一个重要生态和经济性状。先前的研究表明，温度在许多树种中起着调节开花时间的关键作用。然而，开花时间的遗传控制是不同步的，在不同的个体树在可比的温度条件下，基本的分子机制主要是要investigated.ResultsIn本研究中，我们分析了转录丰度在雄性球果和针叶6个早期花粉脱落树（EPs）和6个相邻的晚期花粉脱落树（LPs）在油松在早春连续三个时间点。我们发现，EP和LP在其雄球花中具有不同的首选转录模块，有趣的是，即使在冬季休眠期间，针叶中的表达模式也始终保持不变。此外，在EP中的优选模式也被其他快速生长的组织所采用，例如伸长新梢。增强核酸合成和抗逆途径在寒冷的条件下，可以促进快速增长，并保持较高的转录activity.ConclusionsDuring寒冷的冬季和早春季节，EPs是更敏感的相对温暖的温度，并表现出较高的转录组活性比LP，表明EPs需要较少的热量积累比LP的花粉脱落。这些结果提供了一个转录组的理解松花粉脱落的时间调节。
BackgroundSeasonal flowering time is an ecologically and economically important trait in temperate trees. Previous studies have shown that temperature in many tree species plays a pivotal role in regulating flowering time. However, genetic control of flowering time is not synchronised in different individual trees under comparable temperature conditions, the underlying molecular mechanism is mainly to be investigated.ResultsIn the present study, we analysed the transcript abundance in male cones and needles from six early pollen-shedding trees (EPs) and six neighbouring late pollen-shedding trees (LPs) inPinus tabuliformisat three consecutive time points in early spring. We found that the EPs and LPs had distinct preferred transcriptional modules in their male cones and, interestingly, the expression pattern was also consistently maintained in needles even during the winter dormancy period. Additionally, the preferred pattern in EPs was also adopted by other fast-growing tissues, such as elongating new shoots. Enhancement of nucleic acid synthesis and stress resistance pathways under cold conditions can facilitate rapid growth and maintain higher transcriptional activity.ConclusionsDuring the cold winter and early spring seasons, the EPs were more sensitive to relatively warmer temperatures and showed higher transcriptomic activity than the LPs, indicating that EPs required less heat accumulation for pollen shedding than LPs. These results provided a transcriptomic-wide understanding of the temporal regulation of pollen shedding in pines.
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