Electrochemical analysis of single nucleotide polymorphisms of p53 gene.
Electrochemical analysis of single nucleotide polymorphisms of p53 gene.
复制标题
p53基因单核苷酸多态性的电化学分析。
DOI：
10.1016/s0039-9140(01)00652-x
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发表时间：
2002
期刊：
影响因子：
6.1
通讯作者：
S. Takenaka
中科院分区：
文献类型：
--
作者：
H. Miyahara;K. Yamashita;Masayuki Kanai;Kazuhiko Uchida;M. Takagi;H. Kondo;S. Takenaka
以二茂铁基萘二亚胺(1)为杂交指示剂，对抑癌基因P53的单核苷酸多态性(SNPs)进行了电化学分析。所研究的SNPs是175、248或273位氨基酸密码子中的G向A的转换，以及72位氨基酸从密码子中的G向C的颠换。因此，携带SNP位点的20-Meric寡核苷酸既被用作样品，也被用作探针，后者固定在电极上。即使在P53基因上一个碱基的差异也会导致1的电流响应的显著差异，并且响应的大小与电极上DNA杂交物的数量有关。此外，用该方法对P72/R72SNP所在的第4外显子的PCR产物进行分析时，对杂合子和纯合子的区分具有一定的精密度。
Single nucleotide polymorphisms (SNPs) of cancer repression gene p53 were analyzed electrochemically with ferrocenyl naphthalene diimide (1) as a hybridization indicator. The SNPs studied were the transition to A from G in the codon for amino acid at positions 175, 248 or 273 and the transversion to C from G in the codon for the amino acid at position 72. Thus, 20-meric oligonucleotides carrying the SNP site were used both as a sample and a probe with the latter immobilized on an electrode. Even one base difference on the p53 gene resulted in a significant difference in the current response of 1 and the magnitude of the response correlated with the amount of the DNA hybrid on the electrode. Moreover, when PCR products of exon 4, on which the P72/R72 SNP resides, of the p53 gene were analyzed by this method, the heterozygote and homozygotes were discriminated with modest precision.
影响因子：
14.9
作者：
Kelley, SO;Boon, EM;Hill, MG
通讯作者：
Hill, MG