A molting-inhibiting hormone-like protein from Pacific white shrimp Litopenaeus vannamei is involved in immune responses.
A molting-inhibiting hormone-like protein from Pacific white shrimp Litopenaeus vannamei is involved in immune responses.
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来自太平洋白虾凡纳滨对虾的一种蜕皮抑制激素样蛋白质参与免疫反应。
DOI：
10.1016/j.fsi.2017.11.031
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Xu Xiaopeng
中科院分区：
文献类型：
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作者：
Zuo Hongliang;Yuan Jia;Niu Shengwen;Yang Linwei;Weng Shaoping;He Jianguo;Xu Xiaopeng
甲壳动物高血糖激素（CHH）家族的蜕皮抑制激素（MIH）是一组与甲壳动物蜕皮和生殖调节有关的神经肽。在这项研究中，一个新的蛋白含有一个典型的甲壳动物神经肽结构域，从凡纳滨对虾。该蛋白与其它对虾类MIH蛋白具有较高的同源性，被命名为类MIH蛋白（MIHL）。在检测的组织中，心脏表达最高水平的MIHL。感染白色斑点综合征病毒（WSSV）、革兰氏阴性菌副溶血性弧菌（Vibro parahaemolyticus）和革兰氏阳性菌金黄色葡萄球菌（Staphylococcus aureus）后，MIHL的表达显著上调，提示MIHL可能参与免疫应答。MIHL启动子含有两个NF-κB结合位点，可受NF-κB家族蛋白Relish调控，但不受Dorsal调控，提示MIHL可能是IMD/Relish通路的效应基因。利用RNAi技术沉默MIHLin基因，可显著下调多种免疫效应基因的表达，提高感染对虾的死亡率。和WSSV及其在组织中的拷贝数。这证实了MIHL在对虾抗病毒和抗菌免疫应答中的作用。
The molting-inhibiting hormones (MIHs) from the crustacean hyperglycemic hormone (CHH) family are a group of neuropeptides that are implicated in regulation of molting and reproduction in crustaceans. In this study, a novel protein containing a typical crustacean neuropeptide domain was identified from Litopenaeus vannamei. The protein showed high homology with other shrimp MIHs and was then designated as a MIH-like protein (MIHL). Among the detected tissues, the heart expressed the highest level of MIHL. The expression of MIHL could be significantly up-regulated after infection with white spot syndrome virus (WSSV), gram-negative bacteriumVibro parahaemolyticusand gram-positive bacterium Staphylococcus aureus, indicating that MIHL could be involved in immune responses. The promoter of MIHL was predicted to contain two NF-κB binding sites and could be regulated by the NF-κB family protein Relish but not Dorsal, suggesting that MIHL could be an effector gene of the IMD/Relish pathway. Silencing of MIHLin vivoby RNAi strategy significantly down-regulated the expression of many immune effector genes and increased the mortalities of shrimp infected byV. parahaemolyticusand WSSV and their copy numbers in tissues. These confirmed that MIHL could play a role in antiviral and antibacterial immune responses in shrimp.