ASSOCIATION OF GLYCOLYTIC ENZYMES WITH STRUCTURAL PROTEINS OF SKELETAL-MUSCLE
ASSOCIATION OF GLYCOLYTIC ENZYMES WITH STRUCTURAL PROTEINS OF SKELETAL-MUSCLE
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DOI：
10.1016/0304-4165(75)90187-7
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发表时间：
1975-01-01
期刊：
影响因子：
--
通讯作者：
MASTERS, CJ
中科院分区：
文献类型：
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作者：
CLARKE, FM;MASTERS, CJ
1. 研究了蛋白浓度和离子强度对单个糖酵解酶对f -肌动蛋白和f -肌动蛋白-色氨酸-肌钙蛋白吸附的影响。2. 在生理离子强度和高蛋白浓度的条件下，明显的关联被证明，并且原肌球蛋白-肌钙蛋白被确定为这些相互作用的重要和广泛的组成部分。3. 在此条件下，磷酸果糖激酶、醛缩酶、丙酮酸激酶、乳酸脱氢酶、甘油醛-3-磷酸脱氢酶和葡萄糖-6-磷酸异构体酶结合较强，而三磷酸异构体酶、磷酸甘油激酶、磷酸甘油变化酶、烯醇化酶和己糖激酶对结构蛋白的吸附较弱。4. 考察了一些参数对吸附现象的影响。Ca2+和果糖1,6 -二磷酸增加了醛缩酶、乳酸脱氢酶和丙酮酸激酶的吸附，而等比例组降低了酶的吸附。5. 骨骼肌的其他主要酶组分中，肌酸激酶、腺苷酸激酶和苹果酸脱氢酶在实验条件下对f -肌动蛋白-原肌球蛋白-肌钙蛋白没有吸附作用。然而，在天冬氨酸转氨酶（NADP）异柠檬酸脱氢酶和α -甘油磷酸脱氢酶的情况下，一些吸附是明显的。6. 这些结果已经讨论了它们的功能意义和酶区隔在细胞中的作用。
1. The effects of protein concentration and ionic strength on the adsorption of the individual glycolytic enzymes to F-actin and F-actin--trypomyosin--troponin have been studied. 2. Appreciable association was demonstrated under conditions of physiological ionic strength and high protein concentration, and tropomyosin--troponin established as an important and generalized component of these interactions. 3. Phosphofructokinase, aldolase, pyruvate kinase, lactate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase and glucose-6-phosphate isomerase were strongly bound under these conditions, while triosephosphate isomerase, phosphoglycerate kinase, phosphoglycerate mutase, enolase and hexokinase displayed less adsorption to the structural proteins. 4. The influence of a number of parameters on the adsorption phenomena was examined. Ca2+ and fructose 1, 6-diphosphate increased the adsorption of aldolase, lactate dehydrogenase and pyruvate kinase, while decreasing the adsorption of the enzymes of the constant-proportion group. 5. Of the other major enzymic components of skeletal muscle, creatine kinase, adenylate kinase and malate dehydrogenase showed no adsorption to F-actin--tropomyosin--troponin under the experimental conditions. Some adsorption was evident, however, in the case of aspartate aminotransferase,(NADP) isocitrate dehydrogenase and alpha-glycerolphosphate dehydrogenase. 6. These results have been discussed in relation to their functional significance and the roles of enzyme compartmentation in the cell.