Altered regulation of nerve growth factor secretion by cultured VSMCs from hypertensive rats.
Altered regulation of nerve growth factor secretion by cultured VSMCs from hypertensive rats.
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高血压大鼠培养的 VSMC 改变了神经生长因子分泌的调节。
DOI：
10.1152/ajpheart.1995.269.2.h621
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Tuttle,JB
中科院分区：
文献类型：
--
作者：
Spitsbergen,JM;Stewart,JS;Tuttle,JB
与血压正常的 Wistar-Kyoto (WKY) 大鼠相比，自发性高血压大鼠 (SHR) 的血管组织表现出神经生长因子 (NGF) 水平增加和交感神经支配密度增加。本研究询问在 SHR 和 WKY 大鼠培养的血管平滑肌细胞 (VSMC) 中，基础 NGF 分泌或由类似于交感神经递质的药物引起的分泌是否不同。 VSMC 在无血清培养基 (SFM) 中维持 72 小时，然后在 4、6、8 和 24 小时处理和取样。使用双位点酶联免疫测定法测定条件培养基中的 NGF。在含血清培养基中生长的培养物中，SHR (19.2 +/- 4.6 pg.well-1.48 h-1) 和 WKY VSMC (16.7 +/- 5.4 pg.we..-1.48 h-1) 分泌的 NGF 相似，而维持在 SFM 中的 SHR VSMC 比 WKY VSMC 分泌更多的 NGF（9.1 +/- 1.9 vs. 2.9 +/- 0.4） pg.well-1.24 h-1，分别）。用去氧肾上腺素 (0.1-10 µM)、神经肽 Y (1-1,000 nM) 或 αβ-亚甲基腺苷 5'-三磷酸（10 和 100 µM）处理培养物对 WKY VSMC 的 NGF 分泌没有影响，但增加了 SHR VSMC 的 NGF 分泌。异丙肾上腺素 (0.1-10 microM) 治疗可减少 WKY VSMC 的 NGF 分泌，但不会减少 SHR VSMC 的 NGF 分泌。这些数据表明，对于 SHR 和 WKY 大鼠培养的 VSMC，交感神经递质受体对 NGF 分泌的调节是不同的。如果体内存在类似的差异，则可以解释观察到的 NGF 水平和交感神经支配的变化。
Vascular tissues from spontaneously hypertensive rats (SHR) exhibit increased nerve growth factor (NGF) levels and increased density of sympathetic innervation compared with those from normotensive Wistar-Kyoto (WKY) rats. The present study asked whether basal NGF secretion or secretion elicited by agents analogous to sympathetic neurotransmitters differ in cultured vascular smooth muscle cells (VSMCs) from SHR and WKY rats. VSMCs were maintained in serum-free medium (SFM) for 72 h and then treated and sampled at 4, 6, 8, and 24 h. Conditioned medium was assayed for NGF using a two-site enzyme-linked immunoassay. NGF secretion by SHR (19.2 +/- 4.6 pg.well-1.48 h-1) and WKY VSMCs (16.7 +/- 5.4 pg.we..-1.48 h-1) was similar in cultures grown in serum-containing medium, whereas SHR VSMCs maintained in SFM secrete more NGF than WKY VSMCs (9.1 +/- 1.9 vs. 2.9 +/- 0.4 pg.well-1.24 h-1, respectively). Treatment of cultures with phenylephrine (0.1-10 microM), neuropeptide Y (1-1,000 nM), or alpha beta-methyleneadenosine 5'-triphosphate (10 and 100 microM) had no effect on NGF secretion by WKY VSMCs, while increasing NGF secretion by SHR VSMCs. Treatment with isoproterenol (0.1-10 microM) decreased NGF secretion by WKY VSMCs but not SHR VSMCs. These data indicate that the regulation of NGF secretion by sympathetic neurotransmitter receptors is different for cultured VSMCs from SHR and WKY rats. If similar differences exist in vivo, they could account for the alterations in NGF levels and sympathetic innervation that are observed.