Gd-loaded-RBCs for the assessment of tumor vascular volume by contrast-enhanced-MRI
Gd-loaded-RBCs for the assessment of tumor vascular volume by contrast-enhanced-MRI
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DOI：
10.1016/j.biomaterials.2015.04.026
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发表时间：
2015-07-01
期刊：
影响因子：
14
通讯作者：
Aime, Silvio
中科院分区：
文献类型：
--
作者：
Ferrauto, Giuseppe;Di Gregorio, Enza;Aime, Silvio
肿瘤区域血管体积分数（v（V））的评估在肿瘤的表征中具有重要意义，并且可以用于监测抗血管生成治疗的结果。磁共振成像的高时空分辨率使其成为活体血管容积变化监测的首选成像方式。获得该信息的常用MRI方法依赖于改变体水弛豫时间的造影剂的施用，但是不幸的是，它们只能提供v（V）的估计值，因为它们没有完全保留在血管空间中。在此，Gd-负载的红细胞（Gd-RBC）被提议作为造影剂，能够提供关于肿瘤血管形成的定量信息。由于Gd-RBC完全保留在血管空间中，因此所提出的方法不会受到与使用细胞外Gd试剂相关的限制，所述细胞外Gd试剂在渗漏的肿瘤血管系统中快速外渗。此外，Gd-RBC的长半衰期和生物相容性允许在其施用后多次重复测量;这确保了在体内评估肿瘤生长期间血管体积变化的可能性。由于这些原因，Gd-RBC可以代表脉管系统的高度生物相容性成像报告物，能够定量评估ROI中血管体积的变化。(C)2015爱思唯尔有限公司版权所有。
The assessment of the fractional vascular volume (v(V)) in the tumor area is of great interest in the characterization of tumor and it can be useful to monitor the outcome of anti-angiogenetic therapies. The high spatial and temporal resolution of Magnetic Resonance Imaging makes it the election imaging modality to monitor in vivo the vascular volume changes. Commonly used MRI methods to obtain this information rely on the administration of contrast agents that modify the bulk water relaxation times but, unfortunately, they can provide only an estimate of v(V) since they are not fully retained in the vascular space. Herein, Gd-loaded Red Blood Cells (Gd-RBCs) are proposed as a contrast agent able to provide quantitative information on tumor vascularization. Being Gd-RBCs fully retained in the vascular space, the proposed method does not suffer for the limitations associated to the use of extracellular Gd-agents that quickly extravasate in the leaky tumor vasculature. Furthermore, the long half-life and biocompatibility of Gd-RBCs allows repeating the measurement many times upon their administration; this ensures the possibility to in vivo evaluate the change of vascular volume during tumor growth. For these reasons, Gd-RBCs may represent a highly biocompatible imaging reporter of vasculature, able to quantitatively assess changes in the vascular volume in the ROI. (C) 2015 Elsevier Ltd. All rights reserved.