Gamma-irradiation and UV-C light-induced lipid peroxidation: a Fourier transform-infrared absorption spectroscopic study.
Gamma-irradiation and UV-C light-induced lipid peroxidation: a Fourier transform-infrared absorption spectroscopic study.
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伽马射线照射和 UV-C 光诱导的脂质过氧化：傅里叶变换红外吸收光谱研究。
DOI：
10.1080/095530097143897
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发表时间：
1997
影响因子：
2.6
通讯作者：
J. Wessels
中科院分区：
文献类型：
--
作者：
R. Kinder;Christine Ziegler;J. Wessels
用干式多层膜的傅里叶变换红外光谱研究了1，2-二甘油酰甘油-3-磷胆碱脂质体的伽马辐射和UV-C光诱导的脂质过氧化反应。将观察到的光谱变化与过氧化氢、共轭双烯和硫代巴比妥酸反应物质(如丙二醛或类丙二醛)的形成结果进行了比较。在辐照后，观察到孤立的顺式C=C-H基团(3010 cm-1)的非对称C-H伸缩振动(va(CH2))强度降低。与va(CH2)吸收的减少直接相关的是不对称磷酸酯伸缩振动(va(P=O))向更小的波数(1260-gt；1244 cm-1)移动，这表明脂质过氧化引起的脂肪酸链的分子变化影响了磷脂在干燥的多层膜中的堆积。此外，在1693 cm~(-1)处还观察到一个新的吸收带，其强度与硫代巴比妥酸反应物质的形成相当，因此归因于α，β不饱和醛的(C=O)伸缩。用电离辐射进行的剂量依赖研究表明，va(CH2)的降低与共轭双烯在234 nm处吸收的增加和氢过氧化物的形成直接相关，这表明3010 cm-1处的吸收完全是由孤立的顺式C=C-H基团引起的，因此受到自由基链式反应的早期阶段的影响。UV-C光诱导的脂质过氧化作用表现出I3010的非线性降低，这与过氧化氢的形成直接相关。观察到的早期共轭双烯饱和归因于共轭双键的早期光解。
Fourier transform-infrared spectroscopy of dry, multibilayer films has been used to study gamma-radiation and UV-C light induced lipid peroxidation in 1,2-dilinoleoyl-sn-glycero-3-phosphocholine liposomes. The observed spectral changes were compared with the results obtained from measurement of hydroperoxides, conjugated dienes and to the formation of thiobarbituric acid reactive substances, such as malondialdehyde (MDA) or MDA-like substances. Upon irradiation a decrease in intensity of the asymmetric C - H stretching vibration (va(CH2)) of the isolated cis C = C - H groups (3010 cm-1) was observed. Directly correlated with the decrease of the va(CH2) absorption was a shift of the asymmetric phosphate ester stretching vibration (va(P = O)) towards smaller wavenumbers (1260-->1244 cm-1), indicating that the lipid peroxidation induced molecular alterations in the fatty acid chains influence the packing of the phospholipids in dry multibilayer films. In addition, the formation of a new absorption band at 1693 cm-1 could be detected, the intensity of which was comparable with the formation of thiobarbituric acid reactive substances and, therefore, attributed to the (C = O) stretching of alpha, beta unsaturated aldehydes. Dose-dependent studies using ionizing radiation showed that the decrease of va(CH2) was directly correlated with an increase in absorption of the conjugated dienes at 234 nm and with the formation of hydroperoxides suggesting that the absorption at 3010 cm-1 is solely due to isolated cis C = C - H groups and hence subject to the early stages of the radical chain reaction. UV-C light induced lipid peroxidation revealed a non-linear decrease of I3010, which was directly correlated with the formation of hydroperoxides. The observed early saturation of the conjugated dienes was attributed to an early photodecomposition of the conjugated double bonds.