Microfilament-organizing centers in areas of cell contact: cytoskeletal interactions during cell attachment and locomotion.
Microfilament-organizing centers in areas of cell contact: cytoskeletal interactions during cell attachment and locomotion.
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细胞接触区域中的微丝组织中心：细胞附着和运动过程中的细胞骨架相互作用。
DOI：
10.1083/jcb.99.1.83s
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发表时间：
1984-07
影响因子：
7.8
通讯作者：
Small, V J
中科院分区：
文献类型：
--
作者：
Geiger, B;Avnur, Z;Rinnerthaler, G;Hinssen, H;Small, V J
在这篇文章中，我们讨论了三个方面的细胞接触的形成：（a）细胞基质的分子结构在细胞与基板的焦点接触，（B）的动态特性的膜和microadhesive相关的蛋白质在接触领域，和（c）参与微管在协调和定向形成新的基板接触在细胞运动。我们发现，不同的微生物学相关蛋白表现出不同的模式与局灶接触：一些蛋白质是专门与局灶接触（黏着斑蛋白和talin）; α-辅肌动蛋白在接触区域中富集，但也存在于沿着应力纤维和板状伪足中;肌动蛋白和细丝蛋白在整个接触区域中检测到，但与相关的应力纤维相比，其量明显减少;并且原肌球蛋白、肌球蛋白和血影蛋白或者不存在于接触区域的内面表面，或者仅以非常少量存在。荧光光漂白恢复与活细胞显微注射荧光标记的肌动蛋白，黏着斑蛋白，和α-辅肌动蛋白进行分析表明，这些蛋白质中的每一个保持一个可溶性细胞质池和膜结合部分之间的动态平衡。黏着斑蛋白和微管蛋白在能动成纤维细胞中的分布与相同细胞的前缘的局部运动的相关性表明，自由端微管延伸到活跃的皱褶区域沿着板状伪足，并且新的含黏着斑蛋白的接触优先在这些突出区域形成。
In this article we discuss three aspects of cell contact formation: (a) the molecular architecture of the cytomatrix in cell-to-substrate focal contacts, (b) the dynamic properties of membrane- and microfilament-associated proteins in the contact areas, and (c) the involvement of microtubules in the coordinated and directed formation of new substrate contacts during cell locomotion. We show that different microfilament-associated proteins exhibit distinct patterns of association with focal contacts: some proteins are specifically associated with focal contacts (vinculin and talin); alpha-actinin is enriched in the contact areas but also is present along the stress fibers and in the lamellipodium; actin and filamin are detected throughout the contact areas but in apparently reduced amounts compared with the associated stress fibers; and tropomyosin, myosin, and spectrin are either absent from the endofacial surfaces of contact areas or are present in only very small amounts. Fluorescence photobleaching recovery analyses performed with living cells microinjected with fluorescently labeled actin, vinculin, and alpha-actinin indicate that each of these proteins maintains a dynamic equilibrium between a soluble cytoplasmic pool and a membrane-bound fraction. Correlation of the distribution of vinculin and tubulin in motile fibroblasts to local movements of the leading edge of the same cells indicates that free-end microtubules extend into actively ruffling areas along the lamellipodium and that new vinculin-containing contacts are preferentially formed in these protruding regions.