A FAMILY OF RAN BINDING-PROTEINS THAT INCLUDES NUCLEOPORINS
A FAMILY OF RAN BINDING-PROTEINS THAT INCLUDES NUCLEOPORINS
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DOI：
10.1073/pnas.92.16.7525
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发表时间：
1995-08-01
影响因子：
11.1
通讯作者：
SERAPHIN, B
中科院分区：
文献类型：
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作者：
DINGWALL, C;KANDELSLEWIS, S;SERAPHIN, B
Ran是一种小的核GTP结合蛋白，对于核蛋白通过核孔复合物的移位是必需的。我们发现，几种蛋白质，包括酿酒酵母Nup 2 p和秀丽隐杆线虫F59A2.1核孔蛋白，含有类似于以前表征的小鼠Ran结合蛋白（REP，称为RBP 1）的结构域。为了检验这种相似性的重要性，我们使用了Nup 2 p的相应结构域和一个假定的S。在Ran结合试验和酵母双杂交系统中，这两种蛋白质都与S. cerevisiae Ran，但只有推定的S.酿酒酵母REP结合人Ran。双杂交分析揭示了Ran-Ran相互作用，酵母和人类Rans可以相互作用。这些数据将Nup 2 p鉴定为核孔复合物中Ran的靶标，表明其在核质转运中的直接作用。我们讨论的可能性，蛋白质窝藏然结合域链接的然GTdR循环在细胞核中的特定功能。
Ran, a small nuclear GTP binding protein, is essential for the translocation of nuclear proteins through the nuclear pore complex. We show that several proteins, including the Saccharomyces cerevisiae Nup2p and Caenorhabditis elegans F59A2.1 nucleoporins, contain domains similar to the previously characterized murine Ran binding protein (REP, termed RBP1). To test the significance of this similarity, we have used the corresponding domains of Nup2p and a putative S. cerevisiae REP in Ran binding assays and the yeast two-hybrid system. Both proteins bind S. cerevisiae Ran, but only the putative S. cerevisiae REP binds human Ran. Two-hybrid analysis revealed Ran-Ran interactions and that yeast and human Rans can interact. These data identify Nup2p as a target for Ran in the nuclear pore complex, suggesting a direct role for it in nuclear-cytoplasmic transport. We discuss the possibility that proteins harboring Ran binding domains link the Ran GTPase cycle to specific functions in the nucleus.