Evaluation of the cold tolerance of Saccharum spontaneum L. clones with different ploidy levels on the basis of morphological and physiological indices
Evaluation of the cold tolerance of Saccharum spontaneum L. clones with different ploidy levels on the basis of morphological and physiological indices
复制标题
基于形态和生理指标评价不同倍性甘蔗无性系的耐寒性
DOI：
10.1111/plb.13110
复制
发表时间：
2020
期刊：
影响因子：
3.9
通讯作者：
Yang Q. -H.
中科院分区：
文献类型：
--
作者：
Yang H. -L.;Yu X. -H.;Wang C. -F.;Yang Y.;Wang X. -H.;Yang Q. -H.
自发糖。是现代甘蔗育种最重要的种质资源之一。探索S的耐寒性。不同倍性水平的自发无性系和耐寒材料的筛选有助于耐寒甘蔗育种的亲本选择。通过形态指标、叶片超微结构和生理指标评价36S的耐寒性。具有不同倍性水平的自发克隆（2n=40、48、54、60、64、78、80、88、92和96）。S的形态指数。不同倍性水平的自发克隆与倍性呈正相关。在低温胁迫下，高倍性无性系的叶绿体和线粒体结构比低倍性无性系受到更严重的破坏。综合各项生理指标评价显示，36S.自发克隆可分为强耐冷型、耐冷型、中度耐冷型和冷敏感型四类。形态指标与耐寒性的相关分析表明，耐寒性与倍性呈显着负相关。在形态和生理指标的基础上，建立了可用于耐寒品种筛选的最优逐步回归方程。建立了自发资源。低倍性自发克隆比高倍性自发克隆更耐冷。克隆12-37、13-10和12-23是强耐寒种质资源，表明这些种质资源在耐寒甘蔗育种方面具有较高的潜力。
Saccharum spontaneumL. is one of the most important germplasm resources for modern sugarcane breeding. Exploring the cold tolerance ofS. spontaneumclones with different ploidy levels and screening for cold‐tolerant material can be helpful in parent selection for breeding cold‐tolerant sugarcane.Morphological indices, leaf ultrastructure and physiological indices were used to evaluate the cold tolerance of 36S. spontaneumclones with different ploidy levels (2n = 40, 48, 54, 60, 64, 78, 80, 88, 92 and 96).The morphological indices ofS. spontaneumclones with different ploidy levels were positively correlated with ploidy. Under low‐temperature stress, the chloroplast and mitochondrial structures of the clones with high ploidy were more severely damaged than were those of clones with low ploidy. A comprehensive evaluation of the physiological indices showed that the 36S. spontaneumclones could be divided into four categories: strongly cold tolerant, cold tolerant, moderately cold tolerant and cold sensitive. Correlation analysis of the morphological indices and cold tolerance revealed a significant negative correlation between cold tolerance and ploidy. On the basis of the morphological and physiological indices, optimal stepwise regression equations that can be used for the selection of cold‐tolerantS. spontaneumresources were established.TheS. spontaneumclones with low ploidy are more cold tolerant than those with high ploidy. Clones 12‐37, 13‐10 and 12‐23 are strongly cold‐tolerant germplasm resources, which suggests these germplasm sources have high potential for use in breeding cold‐tolerant sugarcane.