Agonistic and antagonistic effects of zearalenone, an etrogenic mycotoxin, on SKN, HHUA, and HepG2 human cancer cell lines.
Agonistic and antagonistic effects of zearalenone, an etrogenic mycotoxin, on SKN, HHUA, and HepG2 human cancer cell lines.
复制标题
玉米赤霉烯酮（一种外源性霉菌毒素）对 SKN、HHUA 和 HepG2 人类癌细胞系的激动和拮抗作用。
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
I. Ishiwata
中科院分区：
文献类型：
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作者：
Withanage Gs;H. Murata;T. Koyama;I. Ishiwata
玉米赤霉烯酮（ZEA）是一种非甾体类雌激素化合物，主要由禾谷镰刀菌（Fusarium graminearium）和黄色镰刀菌（Fusarium culmorum）产生，广泛存在于世界各地的寄主植物和土壤残体中。ZEA通常对动物不致命，但对畜牧业生产者很重要，因为其高雌激素效应对动物的繁殖性能有不利影响。有人认为ZEA可能参与人类乳腺恶性肿瘤和女性生殖道肿瘤的进展。用MTT法研究了ZEA及其代谢产物α-玉米赤霉烯醇和17-β-雌二醇对SKN、HHUA和HepG 2细胞的毒性和刺激作用。一般而言，两种浓度的17-β-雌二醇（100 M和10 nM）对SKN和HHUA细胞培养物均具有毒性。ZEA和α-玉米赤霉烯醇均刺激SKN和HHUA细胞增殖。在HepG 2细胞上，较低浓度（10 nM）的17-β-雌二醇和较高浓度（100 μ M）的ZEA表现出毒性作用，而用较高浓度的17-β-雌二醇和较低浓度的ZEA处理没有表现出毒性作用。当细胞培养物在雌二醇或霉菌毒素处理之前与ICI 182，780（一种合成雌激素受体阻断剂）预孵育时，观察到剂量依赖性拮抗作用。
Zearalenone (ZEA) is a nonsteroidal estrogenic compound mainly produced by the molds Fusarium graminearium and Fusarium culmorum found in a variety of host plants and soil debris around the world. ZEA is usualy non-lethal to animals but is important to livestock producers because its hyperestrogenic effects adversely influence the reproductive performance of animals. There have been suggestions of possible involvement of ZEA in the progression of breast malignancies and tumors of the female reproductive tract in humans. The toxic or stimulatory effects of ZEA and its metabolites alpha-zearalenol and 17-beta-estradiol on SKN, HHUAand HepG2 cells were studied using rapid colorimetric MTT assay. In general, both concentrations of 17-beta-estradiol (100M and 10 nM) were toxic to SKN and HHUA cell cultures. Both ZEA and alpha-zearalenol stimulated the proliferation of SKN and HHUA cells. On HepG2 cells, lower concentrations (10 nM) of 17-beta-estradiol and higher concentrations (100 microM) of ZEA exhibited toxic effects, whereas treatment with higher concentrations of 17-beta-estradiol and lower concentration of ZEA did not show toxic effects. A dose dependent antagonistic effect was observed when the cell cultures were pre-incubated with ICI 182,780, a synthetic estrogen receptor blocker, before estradiol or mycotoxin treatments.