Prophages of Staphylococcus aureus Newman and their contribution to virulence
Prophages of Staphylococcus aureus Newman and their contribution to virulence
复制标题
DOI：
10.1111/j.1365-2958.2006.05441.x
复制
发表时间：
2006-11-01
影响因子：
3.6
通讯作者：
Schneewind, Olaf
中科院分区：
文献类型：
--
作者：
Bae, Taeok;Baba, Tadashi;Schneewind, Olaf
在金黄色葡萄球菌（Staphylococcus aureus）纽曼（Newman）（一种人类临床分离株）的基因组中鉴定出四种前噬菌体（phi NM 1 -4）。phi NM 1、phi NM 2和phi NM 4是虹吸病毒科的成员，在葡萄球菌染色体中的不同位点（poA，isdB和geh的下游）插入。φ NM 3是一种β-溶血素（hlb）转化噬菌体，除了其它毒力基因外，还编码先天免疫应答（sea、sak、chp和scn）的调节剂。phi NM 1、phi NM 2和phi NM 4的复制发生在培养物中和动物感染期间，而没有观察到phi NM 3原噬菌体复制。从染色体上切除原核细胞，S.缺乏phi NM 3或phi NM 1、phi NM 2和phi NM 4的金黄色葡萄球菌变体在脓肿形成的鼠模型中显示器官特异性毒力缺陷。S.缺乏所有四种前噬菌体的金黄色葡萄球菌纽曼不能引起疾病，从而揭示了前噬菌体对葡萄球菌感染的发病机理的重要贡献。
Four prophages (phi NM1-4) were identified in the genome of Staphylococcus aureus Newman, a human clinical isolate. phi NM1, phi NM2 and phi NM4, members of the siphoviridae family, insert at different sites (poiA, downstream of isdB and geh) in the staphylococcal chromosome. phi NM3, a beta-haemolysin (hlb) converting phage, encodes modulators of innate immune responses (sea, sak, chp and scn) in addition to other virulence genes. Replication of phi NM1, phi NM2 and phi NM4 occurs in culture and during animal infection, whereas phi NM3 prophage replication was not observed. Prophages were excised from the chromosome and S. aureus variants lacking phi NM3 or phi NM1, phi NM2 and phi NM4 displayed organ specific virulence defects in a murine model of abscess formation. S. aureus Newman lacking all four prophages was unable to cause disease, thereby revealing essential contributions of prophages to the pathogenesis of staphylococcal infections.