MicroRNA Dynamics and Functions During Arabidopsis Embryogenesis
MicroRNA Dynamics and Functions During Arabidopsis Embryogenesis
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DOI：
10.1105/tpc.19.00395
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发表时间：
2019-12-01
期刊：
影响因子：
11.6
通讯作者：
Nodine, Michael D.
中科院分区：
文献类型：
--
作者：
Plotnikova, Alexandra;Kellner, Max J.;Nodine, Michael D.
微小RNA（microRNAs，miRNAs）是一类短的非编码RNA，在植物和动物中介导靶基因转录的抑制。虽然miRNAs在植物发育过程中是必需的，但关于它们的胚胎功能知之甚少。为了系统地表征拟南芥（Arabidopsis thaliana）中的胚胎miRNAs，我们开发或应用了基于高通量测序的方法来分析数百种miRNAs和整个胚胎发生过程中的相关靶标。我们发现了数十种动态切割和抑制靶转录物的miRNA，其中包括30种编码转录因子的miRNA。转录组分析表明，这些miRNA：目标相互作用对胚胎基因表达程序有深远的影响。此外，我们证明了六个转录因子的miRNA介导的抑制是单独需要的各种胚胎细胞谱系的正确分裂模式。这些数据表明，多个转录因子的miRNA指导的抑制是至关重要的植物体计划的建立，他们提供了一个基础，以进一步研究miRNA如何有助于这些初始的细胞分化事件。
MicroRNAs (miRNAs) are short noncoding RNAs that mediate the repression of target transcripts in plants and animals. Although miRNAs are required throughout plant development, relatively little is known regarding their embryonic functions. To systematically characterize embryonic miRNAs in Arabidopsis (Arabidopsis thaliana), we developed or applied high-throughput sequencing-based methods to profile hundreds of miRNAs and associated targets throughout embryogenesis. We discovered dozens of miRNAs that dynamically cleave and repress target transcripts, including 30 that encode transcription factors. Transcriptome analyses indicated that these miRNA:target interactions have profound effects on embryonic gene expression programs. Moreover, we demonstrated that the miRNA-mediated repression of six transcription factors are individually required for proper division patterns of various embryonic cell lineages. These data indicate that the miRNA-directed repression of multiple transcription factors is critically important for the establishment of the plant body plan, and they provide a foundation to further investigate how miRNAs contribute to these initial cellular differentiation events.