PIWI proteins are essential for early Drosophila embryogenesis.
PIWI proteins are essential for early Drosophila embryogenesis.
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DOI：
10.1016/j.ydbio.2013.10.017
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发表时间：
2014-01-15
影响因子：
2.7
通讯作者：
Lin, Haifan
中科院分区：
文献类型：
--
作者：
Mani, Sneha Ramesh;Megosh, Heather;Lin, Haifan
PIWI蛋白是ARGONAUTE/PIWI蛋白家族的一个亚家族，参与转录和转录后基因调控以及由小的非编码RNA（尤其是piRNA）介导的转座子沉默。尽管已知这些蛋白质是种系发育所需的，但它们的体细胞功能仍然难以捉摸。在这里，我们研究了所有三个PIWI蛋白质在果蝇的母体功能; Piwi，Aubergine（Aub）和Argonaute 3（Ago 3）在早期胚胎发生。在合胞体胚胎中，Piwi显示出依赖于胚胎阶段的定位模式。Piwi在有丝分裂周期1-10期间定位于细胞质中。在周期11和14之间，Piwi在有丝分裂期间保留在细胞质中，但在间期期间进入体细胞核。在第14周期之后，它会留在细胞核中。Aub和Ago 3在第1-14周期呈弥漫性胞质。三种PIWI蛋白中的任何一种被母体耗尽的胚胎显示出严重的有丝分裂缺陷，包括异常的染色体和核形态、细胞周期停滞、不同步的核分裂和异常的核迁移。此外，所有三种PIWI蛋白都是有丝分裂机器组装和有丝分裂进展所必需的。母体PIWI蛋白耗尽的胚胎也表现出染色质组织异常。这些观察结果表明，母体Piwi，Aub和Ago 3在早期胚胎发生过程中的染色质结构和细胞周期进程的维持中起着至关重要的作用，染色质完整性受损可能是观察到的有丝分裂缺陷的原因。我们的研究表明PIWI蛋白在体细胞发育的第一阶段的基本功能。
PIWI proteins, a subfamily of the ARGONAUTE/PIWI protein family, have been implicated in transcriptional and posttranscriptional gene regulation and transposon silencing mediated by small non-coding RNAs, especially piRNAs. Although these proteins are known to be required for germline development, their somatic function remains elusive. Here, we examine the maternal function of all three PIWI proteins in Drosophila; Piwi, Aubergine (Aub) and Argonaute3 (Ago3) during early embryogenesis. In syncytial embryos, Piwi displays an embryonic stage-dependent localization pattern. Piwi is localized in the cytoplasm during mitotic cycles 1-10. Between cycles 11 and 14, Piwi remains in the cytoplasm during mitosis but moves into the somatic nucleus during interphase. Beyond cycle 14, it stays in the nucleus. Aub and Ago3 are diffusely cytoplasmic from cycle 1-14. Embryos maternally depleted of any one of the three PIWI proteins display severe mitotic defects, including abnormal chromosome and nuclear morphology, cell cycle arrest, asynchronous nuclear division and aberrant nuclear migration. Furthermore, all three PIWI proteins are required for the assembly of mitotic machinery and progression through mitosis. Embryos depleted of maternal PIWI proteins also exhibit chromatin organization abnormalities. These observations indicate that maternal Piwi, Aub and Ago3 play a critical role in the maintenance of chromatin structure and cell cycle progression during early embryogenesis, with compromised chromatin integrity as a possible cause of the observed mitotic defects. Our study demonstrates the essential function of PIWI proteins in the first phase of somatic development.
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影响因子：
4.5
作者：
Khurana JS;Xu J;Weng Z;Theurkauf WE
通讯作者：
Theurkauf WE
影响因子：
4
作者：
Heit, Ryan;Rattner, Jerome B.;Hendzel, Michael J.
通讯作者：
Hendzel, Michael J.
影响因子：
9.2
作者：
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Schüpbach, T
影响因子：
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作者：
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通讯作者：
González-Reyes, A
影响因子：
9.2
作者：
Megosh, Heather B.;Cox, Daniel N.;Lin, Haifan
通讯作者：
Lin, Haifan