SWI1 is required for meiotic chromosome remodeling events
SWI1 is required for meiotic chromosome remodeling events
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DOI：
10.1093/mp/ssn030
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发表时间：
2008-07-01
期刊：
影响因子：
27.5
通讯作者：
Makaroff, Christopher A.
中科院分区：
文献类型：
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作者：
Boateng, Kingsley A.;Yang, Xiaohui;Makaroff, Christopher A.
拟南芥dsy 10突变体先前被鉴定为在减数分裂染色体的联会中有缺陷，导致雄性和雌性不育。我们在这里报告的突变的分子分析，并表明它代表了T-DNA插入SWI 1基因的第三外显子。现在已经在SWI 1中鉴定出四种突变，其中几种表现出不同的表型。例如，swi 1 -1和dyad突变只影响大孢子母细胞的减数分裂，而swi 1 -2和dsy 10突变则阻断雄性和雌性减数分裂。此外，作为一个详细的细胞学特征的dsy 10的性母细胞的一部分，我们确定了几个差异，在男性减数分裂之间的swi 1 -2和dys 10突变体，包括轴向元素的形成，分布的凝聚蛋白和姐妹染色单体凝聚力的过早损失的时间的变化。我们证明，dsy 10代表一个完整的功能丧失突变，而SWI 1的截短形式在减数分裂过程中表达swi 1 -2植物。我们进一步表明，dys 10性母细胞表现出改变修改组蛋白模式，包括乙酰化组蛋白H3和二甲基化组蛋白H3-赖氨酸4。
The Arabidopsis dsy10 mutant was previously identified as being defective in the synapsis of meiotic chromosomes resulting in male and female sterility. We report here the molecular analysis of the mutation and show that it represents a T-DNA insertion in the third exon of the SWI1 gene. Four mutations have now been identified in SWI1, several of which exhibit different phenotypes. For example, the swi1-1 and dyad mutations only affect meiosis in megasporocytes, while the swi1-2 and dsy10 mutations block both male and female meiosis. Furthermore, as part of a detailed cytological characterization of dsy10 meiocytes, we identified several differences during male meiosis between the swi1-2 and dys10 mutants, including variations in the formation of axial elements, the distribution of cohesin proteins and the timing of the premature loss of sister chromatid cohesion. We demonstrate that dsy10 represents a complete loss-of-function mutation, while a truncated form of SWI1 is expressed during meiosis in swi1-2 plants. We further show that dys10 meiocytes exhibit alterations in modified histone patterns, including acetylated histone H3 and dimethylated histone H3-Lysine 4.