Shifts in Diet from High Fat to High Carbohydrate Improved Levels of Adipokines and Pro-inflammatory Cytokines in Mice Fed a High-fat Diet
Shifts in Diet from High Fat to High Carbohydrate Improved Levels of Adipokines and Pro-inflammatory Cytokines in Mice Fed a High-fat Diet
复制标题
DOI：
10.1507/endocrj.k09e-046
复制
发表时间：
2010-01-01
影响因子：
2
通讯作者：
Choue, Ryowon
中科院分区：
文献类型：
--
作者：
Lee, In Seok;Shin, Gina;Choue, Ryowon
高脂饮食导致脂肪组织(AT)扩张，其特征是慢性低度炎症。AT是脂肪因子和促炎细胞因子的重要来源。本研究的目的是评估从高脂饮食向高碳水化合物(CHO)饮食转变对喂食高脂饮食的小鼠血液中脂肪因子和促炎细胞因子水平的影响。6周龄雄性C57BL/6小鼠以高脂饮食(总热量的40%)喂养9周，诱导肥胖，然后转为高CHO饮食(总热量的70%)，持续3周。测量体重、脏器重量以及血脂水平。分析血清脂肪因子和促炎性细胞因子水平。将饮食从高脂肪转变为高CHO显著降低了体重、脂肪组织和肝脏重量(p<；0.05)。血脂水平(甘油三酯、总胆固醇、低密度脂蛋白胆固醇)降低。在饮食转变为高CHO饮食后，瘦素和抵抗素水平显著下降(p<；0.05)。然而，脂联素浓度没有变化。高CHO饮食也能显著降低IL-6水平(p<；0.05)。高CHO饮食可显著提高血清IL-13水平(p<；0.05)。此外，高碳水化合物饮食的小鼠在转入高脂肪饮食后，血清中的肿瘤坏死因子-α水平和培养上清液中的白介素I-β浓度显著增加。血清IL-4和培养上清液水平无明显变化。总而言之，通过从高脂肪饮食转向高碳水化合物饮食来减轻体重和脂肪组织，有效地改善了喂食高脂肪饮食的小鼠的低级别炎症状态。特别是，体重的减轻与瘦素、抵抗素和IL-6的水平有关。
High-fat diets induce an expansion of the adipose tissue (AT) that can be characterized by chronic low-grade inflammation. AT is an important source of adipokines and pro-inflammatory cytokines. The purpose of this study was to evaluate the effects of a shift from a high-fat diet to high-carbohydrate (CHO) diet on the blood levels of adipokines and pro-inflammation cytokines in mice fed a high-fat diet. Six-week-old male C57BL/6 mice were fed a high-fat diet (40% of the total calories) for 9 weeks to induce obesity, and then the diet was shifted to a high CHO diet (70% of the total calories) for 3 weeks. Body weight and organ weight as well as blood lipid levels were measured. The serum levels of adipokines and pro-inflammatory cytokines were analyzed. Shifting the diet from high fat to high CHO decreased significantly body weight, adipose tissues, and liver weight (p < 0.05). The lipid blood levels (TG, Total-chol, and LDL-chol) decreased. The leptin and resistin blood levels significantly decreased after the diet was shifted to a high-CHO diet (p < 0.05). however, the adiponectin concentrations did not change. The IL-6 levels were also significantly decreased by the high-CHO diet (p < 0.05). The IL-13 serum levels were significantly increased by the high-CHO diet (p < 0.05). Further, the serum levels of the TNF-alpha and supernatant IL-I beta concentrations in mice fed a high-carbohydrate diet were significantly increased after the mice were shifted to a high-fat diet. On the other hand, the serum IL-4 and supernatant levels did not change. Conclusively, reduction of body weight and adipose tissues through shifts from a high-fat diet to a high-carbohydrate diet effectively improved low-grade inflammation states in mice fed a high-fat diet. Particularly, the reduction of body weight was associated with the levels of leptin, resistin, and IL-6.