Three-dimensional reconstructions of the internal structures of haustoria in parasitic Orobanchaceae
Three-dimensional reconstructions of the internal structures of haustoria in parasitic Orobanchaceae
复制标题
寄生列当科吸器内部结构的三维重建
DOI：
10.1101/2020.05.07.083055
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发表时间：
2020
期刊：
影响因子：
7.4
通讯作者：
Yoshida Satoko
中科院分区：
文献类型：
--
作者：
Masumoto Natsumi;Suzuki Yuki;Cui Songkui;Wakazaki Mayumi;Sato Mayuko;Kumaishi Kie;Shibata Arisa;Furuta Kaori M.;Ichihashi Yasunori;Shirasu Ken;Toyooka Kiminori;Sato Yoshinobu;Yoshida Satoko
寄生植物通过形成吸器感染其他植物，吸器是由几种细胞类型组成的专门的多细胞器官，每种细胞类型具有独特的形态特征和与寄生相关的生理作用。因此，了解细胞类型的空间组织对于阐明吸器的功能具有重要意义。在这里，我们报告了一个三维（3-D）重建吸器从列当科物种，专性寄生Striga hermonthica感染水稻和兼性寄生Phtheirospermum japonicum感染拟南芥。我们的图像揭示了吸器内多种细胞类型的空间排列及其与宿主根的相互作用。吸器的三维内部结构突出了两种寄生虫之间的差异，特别是在木质部连接网站与主机。我们的研究提供了结构的见解器官之间如何相互作用的主机和寄生plants.One-sentence summaryThree-dimensional图像重建是用来可视化的空间组织的细胞类型的吸器的寄生植物，特别是参考他们与主机根的相互作用。
Parasitic plants infect other plants by forming haustoria, specialized multicellular organs consisting of several cell types each of which has unique morphological features and physiological roles associated with parasitism. Understanding the spatial organization of cell types is, therefore, of great importance in elucidating the functions of haustoria. Here, we report a three-dimensional (3-D) reconstruction of haustoria from two Orobanchaceae species, the obligate parasiteStriga hermonthicainfecting rice and the facultative parasitePhtheirospermum japonicuminfectingArabidopsis. Our images reveal the spatial arrangements of multiple cell types inside haustoria and their interaction with host roots. The 3-D internal structures of haustoria highlight differences between the two parasites, particularly at the xylem connection site with the host. Our study provides structural insights into how organs interact between hosts and parasitic plants.One-sentence summaryThree-dimensional image reconstruction was used to visualize the spatial organization of cell types in the haustoria of parasitic plants with special reference to their interaction with host roots.