Biological activity of diuretic factors on the anterior midgut of the blood-feeding bug, Rhodnius prolixus
Biological activity of diuretic factors on the anterior midgut of the blood-feeding bug, Rhodnius prolixus
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DOI：
10.1016/j.ygcen.2009.01.025
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发表时间：
2009-05-15
影响因子：
2.7
通讯作者：
Orchard, Ian
中科院分区：
文献类型：
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作者：
Brugge, Victoria Te;Ianowski, Juan P.;Orchard, Ian
在五岁的长尾红鲌（Rhodnius prolixus）中，探测宿主和摄取血液会导致一系列紧密结合的事件，包括唾液腺分泌、腹部角质层的塑化、离子和水通过前中肠（嗉囊）和马尔比氏小管（快速产生尿液）的运动增加，以及后肠定期排出尿液。在这项研究中，我们关注的是前中肠在餐后快速利尿中的作用。在进食过程中，大量的血粉被泵入前中肠，然后通过利尿处理并储存起来，直到被消化。中肠前部活动变化迅速。在开始进食的几分钟内，前中肠收缩频率增加，随着盐和水穿过前中肠上皮进入血淋巴，利尿开始。血清素是一种利尿激素，已知在中肠前部的生理活动中起作用，我们有兴趣进一步探索血清素和其他利尿肽的作用。我们测试了几种多肽的活性，包括长毛霉降钙素样利尿激素（Rhopr-DH31）、内腺虫病DH （Zoone-DH）的促肾上腺皮质激素释放因子（CRF）样肽和白蛉的激肽。白细胞介素1 （LK 1）。已知这些多肽家族存在于长毛X的中枢神经系统中，是进食后释放到血淋巴中的神经激素，并已被证明对多种参与进食后利尿的组织有活性。我们在这里显示血清素和zone - dh都增加了前中肠的cAMP含量和血清素。zone - dh和cAMP类似物增加了前中肠对水的吸收，增加了短路电流和电压，同时降低了上皮间的电阻。而LK1和Rhopr-DH31对吸收和短路电流无明显增加作用。LK1确实显著降低了前中肠上皮的阻力和过皮上皮电压。所有研究的因素都增加了前中肠收缩的频率。(C) 2009爱思唯尔公司版权所有。
Probing of a host and ingestion of a blood-meal in a fifth instar Rhodnius prolixus results in a cascade of tightly integrated events, including salivary gland secretion, plasticisation of the abdominal cuticle, increased ion and water movement across the anterior midgut (crop) and Malpighian tubules (which rapidly produce urine) and the regular expulsion of urine from the hindgut. In this study we have focussed on the role of the anterior midgut during the rapid postprandial diuresis. The huge blood-meal is pumped into the anterior midgut, during feeding, then modified by diuresis and stored until it is digested. Changes in the anterior midgut activity are rapid. Within minutes of the commencement of feeding there is an increase in the frequency of anterior midgut contractions and diuresis begins with the movement of salt and water across the epithelium of the anterior midgut into the haemolymph.While serotonin, a diuretic hormone in R prolixus, is known to play a role in the physiological activity of the anterior midgut, we were interested in exploring further the role of serotonin, and other diuretic peptides. We have tested the activity of several peptides, including R. prolixus calcitonin-like diuretic hormone (Rhopr-DH31), corticotropin-releasing factor (CRF)-like peptide from Zootermopsis nevadensis DH (Zoone-DH) and a kinin from Leucophaea maderae. Leucokinin 1 (LK 1). These peptides families are known to be present in the central nervous system of X prolixus, are putative neurohormones released into the haemolymph after the start of feeding, and have been shown to have activity on a variety of tissues involved in post-feeding diuresis. We show here that both serotonin and Zoone-DH increase the cAMP content of the anterior midgut and that serotonin. Zoone-DH and cAMP analogues increase absorption of water from the anterior midgut, increase the short circuit current and voltage, while decreasing the resistance across the epithelium. While LK1 and Rhopr-DH31 do not significantly increase absorption, or short circuit current. LK1 does significantly decrease the resistance and transepithelial voltage of the anterior midgut epithelium. All of the factors studied increase the frequency of contractions of the anterior midgut. (C) 2009 Elsevier Inc. All rights reserved.