Gold NanoStoves for microsecond DNA melting analysis
Gold NanoStoves for microsecond DNA melting analysis
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DOI：
10.1021/nl073028i
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发表时间：
2008-02-01
期刊：
影响因子：
10.8
通讯作者：
Feldmann, Jochen
中科院分区：
文献类型：
--
作者：
Stehr, Joachim;Hrelescu, Calin;Feldmann, Jochen
在传统的DNA熔化试验中，含有DNA的溶液的温度缓慢升高。相比之下，我们使用300ns激光脉冲来快速加热DNA结合的金纳米颗粒聚集体。我们发现，双链DNA在微秒的时间尺度上融化，导致金纳米粒子聚集体在毫秒的时间尺度上解体。一个完全匹配的DNA序列和一个点突变的DNA序列可以在不到一毫秒的时间内清楚地区分出来，即使是在1:1的混合物中。
In traditional DNA melting assays, the temperature of the DNA-containing solution is slowly ramped up. In contrast, we use 300 ns laser pulses to rapidly heat DNA bound gold nanoparticle aggregates. We show that double-stranded DNA melts on a microsecond time scale that leads to a disintegration of the gold nanoparticle aggregates on a millisecond time scale. A perfectly matching and a point-mutated DNA sequence can be clearly distinguished in less than one millisecond even in a 1:1 mixture of both targets.