Effects of Vibration Therapy on Immobilization-Induced Hypersensitivity in Rats
Effects of Vibration Therapy on Immobilization-Induced Hypersensitivity in Rats
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DOI：
10.2522/ptj.20140137
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发表时间：
2015-07-01
期刊：
影响因子：
3.2
通讯作者：
Origuchi, Tomoki
中科院分区：
文献类型：
--
作者：
Hamaue, Yohei;Nakano, Jiro;Origuchi, Tomoki
背景。石膏固定会引起机械过敏，从而影响康复。尽管振动疗法可以减轻各种类型的疼痛，但振动是否可以减轻固定引起的超敏反应仍不清楚。 目的。本研究的目的是探讨振动疗法对固定引起的超敏反应的预防和治疗效果。设计。该研究的实验设计涉及在模型大鼠中进行行为、组织学和免疫组织化学研究。方法。使用 35 只 Wistar 大鼠（8 周龄，均为雄性）。 30只大鼠右踝关节石膏固定8周，另5只大鼠作为对照。将固定大鼠随机分为以下3组：（1）仅固定组（Im，n=10）； (2)振动治疗组1，在固定开始后立即开始振动治疗(Im+Vib1，n=10)； (3)振动治疗组2，在固定开始4周后开始振动治疗(Im+Vib2，n=10)。对后爪施加振动。测量后爪皮肤的机械超敏性和表皮厚度。为了研究中枢敏化，分析了脊髓和背根神经节（DRG）中降钙素基因相关肽（CGRP）的表达。结果。 Im+Vib1 组中固定诱导的超敏反应受到抑制，但 Im+Vib2 组中则没有。中枢敏化（表现为脊髓中 CGRP 表达的增加和 DRG 中 CGRP 阳性神经元面积的大小）仅在 Im+Vib1 组中受到抑制。表皮厚度不受振动刺激的影响。局限性。这项研究的局限性在于，结果仅限于动物模型，不能推广到人类。结论。数据表明，在固定的早期阶段开始振动治疗可能会抑制固定引起的超敏反应的发展。
Background. Cast immobilization induces mechanical hypersensitivity, which disturbs rehabilitation. Although vibration therapy can reduce various types of pain, whether vibration reduces immobilization-induced hypersensitivity remains unclear.Objective. The purpose of this study was to investigate the preventive and therapeutic effects of vibration therapy on immobilization-induced hypersensitivity.Design. The experimental design of the study involved conducting behavioral, histological, and immunohistochemical studies in model rats.Methods. Thirty-five Wistar rats (8 weeks old, all male) were used. The right ankle joints of 30 rats were immobilized by plaster cast for 8 weeks, and 5 rats were used as controls. The immobilized rats were divided randomly into the following 3 groups: (1) immobilization-only group (Im, n=10); (2) vibration therapy group 1, for which vibration therapy was initiated immediately after the onset of immobilization (Im+Vib1, n=10); and (3) vibration therapy group 2, for which vibration therapy was initiated 4 weeks after the onset of immobilization (Im+Vib2, n=10). Vibration was applied to the hind paw. The mechanical hypersensitivity and epidermal thickness of the hind paw skin were measured. To investigate central sensitization, calcitonin gene-related peptide (CGRP) expression in the spinal cord and dorsal root ganglion (DRG) was analyzed.Results. Immobilization-induced hypersensiiivity was inhibited in the Im+Vib1 group but not in the Im+Vib2 group. Central sensitization, which was indicated by increases in CGRP expression in the spinal cord and the size of the area of CGRP-positive neurons in the DRG, was inhibited in only the Im+Vib1 group. Epidermal thickness was not affected by vibration stimulation.Limitations. A limitation of this study is that the results were limited to an animal model and cannot be generalized to humans.Conclusions. The data suggest that initiation of vibration therapy in the early phase of immobilization may inhibit the development of immobilization-induced hypersensitivity.