Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase
Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase
复制标题
DOI：
10.1046/j.1365-313x.1998.00063.x
复制
发表时间：
1998-03-01
期刊：
影响因子：
7.2
通讯作者：
Gancedo, C
中科院分区：
文献类型：
--
作者：
Blázquez, MA;Santos, E;Gancedo, C
拟南芥cDNA克隆，AtTPS 1，其编码海藻糖-6-磷酸合酶被分离。这种蛋白质的身份得到了结构和功能证据的支持。一方面，AtTPS 1编码蛋白的预测序列与不同生物的海藻糖-6-磷酸脱氢酶具有高度的相似性。另一方面，AtTPS 1 cDNA在酵母tps 1突变体中的表达恢复了其合成海藻糖的能力，并抑制了与缺乏海藻糖-6-磷酸有关的生长缺陷。基因组组织和表达分析表明，AtTPS 1是一个单拷贝基因，并在非常低的水平上组成型表达。
An Arabidopsis thaliana cDNA clone, AtTPS1, that encodes a trehalose-6-phosphate synthase was isolated. The identity of this protein is supported by both structural and functional evidence. On one hand, the predicted sequence of the protein encoded by AtTPS1 showed a high degree of similarity with trehalose-6-phosphate synthases of different organisms. On the other hand, expression of the AtTPS1 cDNA in the yeast tps1 mutant restored its ability to synthesize trehalose and suppressed its growth defect related to the lack of trehalose-6-phosphate. Genomic organization and expression analyses suggest that AtTPS1 is a single-copy gene and is expressed constitutively at very low levels.