Antibacterial Activity and Mechanism of Action of Chlorogenic Acid
Antibacterial Activity and Mechanism of Action of Chlorogenic Acid
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DOI：
10.1111/j.1750-3841.2011.02213.x
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发表时间：
2011-08-01
影响因子：
3.9
通讯作者：
Wang, Zhouping
中科院分区：
文献类型：
--
作者：
Lou, Zaixiang;Wang, Hongxin;Wang, Zhouping
在本研究中，评估了绿原酸对细菌的抗菌活性和作用机制。最低抑菌浓度（MIC）值数据表明，绿原酸有效抑制所有受试细菌病原体的生长，MIC值范围为20～80μg/mL。绿原酸对病原菌作用方式的研究表明，绿原酸显着增加外膜和质膜的通透性，导致屏障功能丧失，甚至引起核苷酸的轻微渗漏。还通过电子显微照片观察到细胞质内容物的泄漏。这些结果支持了我们的假设，即绿原酸与外膜结合，破坏细胞膜，耗尽细胞内电位，释放细胞质大分子，从而导致细胞死亡。
In this study, the antibacterial activity and mechanism of action of chlorogenic acid against bacteria were assessed. The data from minimum inhibitory concentration (MIC) values showed that chlorogenic acid effectively inhibited the growth of all tested bacterial pathogens, and the MIC values were ranging from 20 to 80 mu g/mL. An investigation into action mode of chlorogenic acid against the pathogen indicated that chlorogenic acid significantly increased the outer and plasma membrane permeability, resulting in the loss of the barrier function, even inducing slight leakage of nucleotide. The leakage of cytoplasmic contents was also observed by electron micrographs. These results supported our hypothesis that chlorogenic acid bound to the outer membrane, disrupted the membrane, exhausted the intracellular potential, and released cytoplasm macromolecules, which led to cell death.