Lysosome activation in peripheral blood mononuclear cells and prognostic significance of circulating LC3B in COVID-19
Lysosome activation in peripheral blood mononuclear cells and prognostic significance of circulating LC3B in COVID-19
复制标题
外周血单核细胞中的溶酶体激活以及循环 LC3B 在 COVID-19 中的预后意义
DOI：
10.1093/bib/bbab043
复制
发表时间：
2021-02-23
影响因子：
9.5
通讯作者：
Xia, Junjie
中科院分区：
文献类型：
--
作者：
Fang, Shisong;Zhang, Lin;Xia, Junjie
2019冠状病毒病（COVID-19）在全球范围内迅速蔓延，导致大量死亡。细胞内冠状病毒复制与自噬体-溶酶体系统失调之间存在机制关系。我们对COVID-19患者的外周血单核细胞（PBMC）进行了转录组分析，并确定了溶酶体途径中基因的异常上调。我们进一步确定了两种循环标志物，即微管相关蛋白1A/1B轻链3B（LC 3B）和（p62/SQSTM 1）p62（两者都依赖于溶酶体降解）在预测需要住院接受补充氧气治疗的COVID-19患者出现中度至重度疾病方面的能力。Logistic回归分析显示，LC 3B与中度至重度COVID-19相关，与年龄、性别和临床风险评分无关。LC 3B浓度降低
Coronavirus disease 2019 (COVID-19) has spread rapidly worldwide, causing significant mortality. There is a mechanistic relationship between intracellular coronavirus replication and deregulated autophagosome-lysosome system. We performed transcriptome analysis of peripheral blood mononuclear cells (PBMCs) from COVID-19 patients and identified the aberrant upregulation of genes in the lysosome pathway. We further determined the capability of two circulating markers, namely microtubule-associated proteins 1A/1B light chain 3B (LC3B) and (p62/SQSTM1) p62, both of which depend on lysosome for degradation, in predicting the emergence of moderate-to-severe disease in COVID-19 patients requiring hospitalization for supplemental oxygen therapy. Logistic regression analyses showed that LC3B was associated with moderate-to-severe COVID-19, independent of age, sex and clinical risk score. A decrease in LC3B concentration