Developmental stage- and spermatogenic cycle-specific expression of transcription factor GATA-1 in mouse Sertoli cells.
Developmental stage- and spermatogenic cycle-specific expression of transcription factor GATA-1 in mouse Sertoli cells.
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DOI：
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发表时间：
1994-07
期刊：
影响因子：
4.6
通讯作者：
K. Yomogida;Haruo Ohtani;Hideo Harigae;Etsuro Ito;Yoshitake Nishimune;J. D. Engel;Masayuki Yamamoto
中科院分区：
文献类型：
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作者：
K. Yomogida;Haruo Ohtani;Hideo Harigae;Etsuro Ito;Yoshitake Nishimune;J. D. Engel;Masayuki Yamamoto
加塔-1是红系特异性基因转录激活的必需因子，并且在小鼠睾丸小管中与生精上皮相邻的离散细胞亚群中也大量表达。在检查正常和生殖系缺陷突变小鼠，我们在这里显示，加塔-1只在小鼠睾丸支持细胞系中表达。加塔-1在支持细胞中的表达与精子发生的第一波同时诱导，并且加塔-1阳性细胞在青春期前睾丸发育期间均匀地分布在所有小管中。然而，加塔-1阳性细胞的数量此后下降，并发现只有在生精小管的外周带在阶段VII，VIII和IX的精子发生在成年小鼠睾丸。相反，突变型W/Wv、jsd/jsd或隐睾小鼠（所有小鼠均缺乏大量生殖细胞）中几乎每个支持细胞都表达加塔-1，因此表明该转录因子的表达受成熟生殖细胞的负调控。这些观察结果表明，转录因子加塔-1是一个发育阶段和生精周期的基因表达的特定调节支持细胞。
GATA-1 is an essential factor for the transcriptional activation of erythroid-specific genes, and is also abundantly expressed in a discrete subset of cells bordering the seminiferous epithelium in tubules of the murine testis. In examining normal and germ-line defective mutant mice, we show here that GATA-1 is expressed only in the Sertoli cell lineage in mouse testis. GATA-1 expression in Sertoli cells is induced concomitantly with the first wave of spermatogenesis, and GATA-1-positive cells are uniformly distributed among all tubules during prepubertal testis development. However, the number of GATA-1-positive cells declines thereafter and were found only in the peripheral zone of seminiferous tubules in stages VII, VIII and IX of spermatogenesis in the adult mouse testis. In contrast, virtually every Sertoli cell in mutant W/Wv, jsd/jsd or cryptorchid mice (all of which lack significant numbers of germ cells) expresses GATA-1, thus showing that the expression of this transcription factor is negatively controlled by the maturing germ cells. These observations suggest that transcription factor GATA-1 is a developmental stage- and spermatogenic cycle-specific regulator of gene expression in Sertoli cells.