A Review of In Vitro Instrumentation Platforms for Evaluating Thermal Therapies in Experimental Cell Culture Models.
A Review of In Vitro Instrumentation Platforms for Evaluating Thermal Therapies in Experimental Cell Culture Models.
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DOI：
10.1615/critrevbiomedeng.2022043455
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发表时间：
2022
影响因子：
--
通讯作者：
Prakash, Punit
中科院分区：
文献类型：
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作者：
Chamani, Faraz;Barnett, India;Pyle, Marla;Shrestha, Tej;Prakash, Punit
热疗法，调节组织温度以获得治疗益处，在临床上用作一系列适应症的辅助或独立治疗方式，并且正在研究其他适应症。在临床和实验环境中进行热治疗期间，时空热分布的监测和控制有助于增加诱导所需生物效应的可能性。体外热剂量学研究为表征细胞对热的生物反应提供了强有力的基础。为了进行准确的体外热分析，样品需要在理想的等温条件下经受均匀加热，理想地立即从基线升高并返回到基线，持续已知的加热持续时间。虽然大多数加热方式以温度控制的方式成功地输送热量，但控制的准确性各不相同。本文综述了体外加热仪器平台的应用概况。调查了各种不同的方法，包括外部热源（即，CO2培养箱、循环水浴、微加热器和微流体装置）到微波介电加热、激光或使用声波。我们讨论了关键的加热参数，包括升温速率（加热阶段），加热精度，复杂性，峰值温度和每种加热方式的技术限制。
Thermal therapies, the modulation of tissue temperature for therapeutic benefit, are in clinical use as adjuvant or stand-alone therapeutic modalities for a range of indications, and are under investigation for others. During delivery of thermal therapy in the clinic and in experimental settings, monitoring and control of spatio-temporal thermal profiles contributes to an increased likelihood of inducing desired bioeffects. In vitro thermal dosimetry studies have provided a strong basis for characterizing biological responses of cells to heat. To perform an accurate in vitro thermal analysis, a sample needs to be subjected to uniform heating, ideally raised from, and returned to, baseline immediately, for a known heating duration under ideal isothermal condition. While most heating modalities successfully delivered the heat in a temperature-controlled manner, the accuracy of control varies. This review presents an applications-based overview of in vitro heating instrumentation platforms. A variety of different approaches are surveyed, including external heating sources (i.e., CO2 incubators, circulating water baths, microheaters and microfluidic devices) to microwave dielectric heating, lasers or the use of sound waves. We discuss critical heating parameters including temperature ramp rate (heat-up phase period), heating accuracy, complexity, peak temperature, and technical limitations of each heating modality.