A quantitative study of the kinetics of blood clearance of P32-labelled Escherichia coli and Staphylococci by the reticuloendothelial system.
A quantitative study of the kinetics of blood clearance of P32-labelled Escherichia coli and Staphylococci by the reticuloendothelial system.
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DOI：
10.1084/jem.110.1.27
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发表时间：
1959-07-01
期刊：
影响因子：
--
通讯作者：
SCHLOSSMAN S
中科院分区：
文献类型：
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作者：
BENACERRAF B;SEBESTYEN MM;SCHLOSSMAN S
1. P32标记的热灭活E.大肠杆菌和葡萄球菌从血液中遵循时间的指数函数，并且细菌主要被肝和脾的RES吞噬。2.等量E.从血液中清除大肠杆菌在兔子体内是快速的，在小鼠体内是缓慢的，似乎与这些动物血清中的抗体水平有关。3.与E.大肠杆菌、葡萄球菌被RES从具有足够水平的抗这些细菌的血清抗体的小鼠的血液中快速有效地清除。4.分别被肝脏或脾脏吞噬的细菌数量取决于细菌的清除率和调理作用的程度。快速清除，良好调理E。大肠杆菌几乎完全由肝脏清除，而吞噬效率较低的细菌也大量由脾脏清除。5. E.大肠杆菌的抗体水平和小鼠体内RES吞噬它们的效率与血清中抗体水平直接相关。6.用S.伤寒杆菌或粘质沙雷氏菌内毒素可提高大肠杆菌的清除率。大肠埃希氏菌抗体水平及血清中大肠埃希氏菌抗体水平。血清中的大肠杆菌。E.内毒素处理的小鼠血清可将大肠杆菌转移到正常小鼠体内。
1. The clearance of P32-labelled heat-killed E. coli and staphylococci from the blood follows an exponential function of the time, and the bacteria are phagocytized principally by the RES of the liver and spleen. 2. The rates of clearance of equivalent number of E. coli from the blood is rapid in rabbits and slow in mice and appears to be related to the level of antibodies in the serum of these animals. 3. Unlike E. coli, staphylococci are cleared rapidly and efficiently by the RES from the blood of mice which have a sufficient level of serum antibody against these bacteria. 4. The numbers of bacteria, phagocytized by the liver or the spleen respectively, depend upon the rate of clearance and the extent of opsonization of the bacteria. Rapidly cleared, well opsonized E. coli are removed almost exclusively by the liver, while less efficiently phagocytized bacteria are also cleared by the spleen in large numbers. 5. The rate of clearance of E. coli and the efficiency with which they are phagocyted by the RES in mice have been shown to be directly related to the level of antibody in the serum. 6. Treatment of mice with S. typhi or Serratia marcescens endotoxins increases the rate of clearance of E. coli from the blood and the level of antibody against E. coli in the serum. The enhanced clearance of E. coli can be transferred to normal mice by the serum of endotoxin-treated mice.