INDUCTION BY ANTIGEN OF INTRATHYMIC APOPTOSIS OF CD4+CD8+TCRLO THYMOCYTES INVIVO
INDUCTION BY ANTIGEN OF INTRATHYMIC APOPTOSIS OF CD4+CD8+TCRLO THYMOCYTES INVIVO
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DOI：
10.1126/science.2125367
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发表时间：
1990-12-21
期刊：
影响因子：
56.9
通讯作者：
LOH, DY
中科院分区：
文献类型：
--
作者：
MURPHY, KM;HEIMBERGER, AB;LOH, DY
为了研究自身反应性T细胞克隆缺失发生的机制，使用肽抗原在体内诱导抗原反应性胸腺细胞缺失。与该肽发生反应的 T 细胞受体 (TCR) 转基因小鼠含有从不成熟表型发展到成熟表型的胸腺细胞。对转基因小鼠腹膜内施用肽抗原导致未成熟的CD4+CD8+TCRlo胸腺细胞的快速缺失。治疗20小时内即可看到皮质胸腺细胞凋亡。这些结果为细胞凋亡在抗原诱导的耐受性发展中的体内作用提供了直接证据。
In order to examine the mechanisms by which clonal deletion of autoreactive T cells occurs, a peptide antigen was used to induce deletion of antigen-reactive thymocytes in vivo. Mice transgenic for a T cell receptor (TCR) that reacts to this peptide contain thymocytes that progress from the immature to the mature phenotype. Intraperitoneal administration of the peptide antigen to transgenic mice results in a rapid deletion of the immature CD4+ CD8+ TCRlo thymocytes. Apoptosis of cortical thymocytes can be seen within 20 hours of treatment. These results provide direct evidence for the in vivo role of apoptosis in the development of antigen-induced tolerance.