Cytogenetic studies on the origin and species differentiation of the Philippine medaka, Oryzias luzonensis
Cytogenetic studies on the origin and species differentiation of the Philippine medaka, Oryzias luzonensis
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菲律宾青鳉Oryzias luzonensis起源和物种分化的细胞遗传学研究
DOI：
10.1111/j.1095-8649.1985.tb04030.x
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发表时间：
1985
影响因子：
2
通讯作者：
H. Uwa
中科院分区：
文献类型：
--
作者：
M. J. Formacion;H. Uwa
通过核型分析和种间杂交研究了菲律宾稻（Oryzias luzonensis）的起源和种间分化。核型为2n = 48，由24对双臂染色体组成（NF = 96）。在三个亚稳心对中发现了特征c带。在卫星亚稳心对的短臂上检测到核仁组织区。估计每个细胞核的dna值为1.9 pg。种间杂交结果表明，O. luzonensis与O. latipes（双臂染色体型）亲缘关系最密切，与O. celebensis（融合染色体型）亲缘关系次之，与O. melastigma和O.javanicus（单臂染色体型）亲缘关系最不密切，这两种植物分别分布在东亚、苏拉威西、西南亚和东南亚西部。吕宋稻的外形也更接近于拉丁稻。根据这些结果，我们得出结论认为，吕宋古树是双臂染色体群的成员，是与拉丁古树最近的近亲。因此，菲律宾medaka似乎是东亚起源，从中国大陆经台湾迁移到吕宋岛北部。
The origin and species differentiation of the Philippine medaka, Oryzias luzonensis, was studied by means of karyotype analyses and interspecific hybridyzations. The karyotype revealed 2n = 48 consisting of 24 pairs of biarmed chromosomes (NF = 96). Characteristic C-bands were found in three sub-metacentric pairs. Nucleolar organizer regions (NORs) were detected on the short arms of the satellited sub-metacentric pair. Estimated DNA-value was 1.9 pg per nucleus. Interspecific crosses revealed that O. luzonensis was allied most closely to O. latipes (biarmed chromosome type), less closely to O. celebensis (fused chromosome type), and least closely to O. melastigma and O.javanicus (monoarmed chromosome type), which are distributed in east Asia, the Sulawesi, and south-west Asia and west side of south-east Asia, respectively. The external appearance of O. luzonensis was also more similar to that of O. latipes. From these results, we concluded that O. luzonensis is a member of the biarmed chromosome group and the nearest relative to O. latipes. Thus, the Philippine medaka seems to be of east Asiatic origin, having migrated to northern Luzon from the China continent via Formosa.