The Det.Belt Server: A Tool to Visualize and Estimate Amphipathic Solvent Belts around Membrane Proteins.
The Det.Belt Server: A Tool to Visualize and Estimate Amphipathic Solvent Belts around Membrane Proteins.
复制标题
DOI：
10.3390/membranes11070459
复制
发表时间：
2021-06-22
期刊：
影响因子：
4.2
通讯作者：
Chaptal V
中科院分区：
文献类型：
--
作者：
Zampieri V;Hilpert C;Garnier M;Gestin Y;Delolme S;Martin J;Falson P;Launay G;Chaptal V
去污剂包裹膜蛋白以形成覆盖蛋白质的疏水部分的带，用于膜插入和与脂质相互作用。形成该带的洗涤剂单体的数量对于研究者来说通常是未知的，除非进行专用的洗涤剂定量，这对于许多项目来说是难以设置的。然而，具有形成带的去污剂的量的近似知识是非常有用的，以更好地掌握与其直接环境相互作用的感兴趣的蛋白质，而不是描绘“裸露的”膜蛋白质。我们创建了Det.Belt服务器来装扮膜蛋白，并在3D中表示由洗涤剂分子包裹在带中制成的块。数据库中包含许多洗涤剂，使研究人员能够通过计算机筛选不同洗涤剂对膜蛋白的影响。洗涤剂的输入数量是可变的，带的快速重新计算用于交互式使用。代表腰带的插图与脚本一起提供，以呈现高质量的3D图像供出版。Det.Belt服务器是生物化学家更好地掌握样品的工具。
Detergents wrap around membrane proteins to form a belt covering the hydrophobic part of the protein serving for membrane insertion and interaction with lipids. The number of detergent monomers forming this belt is usually unknown to investigators, unless dedicated detergent quantification is undertaken, which for many projects is difficult to setup. Yet, having an approximate knowledge of the amount of detergent forming the belt is extremely useful, to better grasp the protein of interest in interaction with its direct environment rather than picturing the membrane protein “naked”. We created the Det.Belt server to dress up membrane proteins and represent in 3D the bulk made by detergent molecules wrapping in a belt. Many detergents are included in a database, allowing investigators to screen in silico the effect of different detergents around their membrane protein. The input number of detergents is changeable with fast recomputation of the belt for interactive usage. Metrics representing the belt are readily available together with scripts to render quality 3D images for publication. The Det.Belt server is a tool for biochemists to better grasp their sample.
登录
查看更多内容
影响因子：
14.9
作者：
wwPDB consortium
通讯作者：
wwPDB consortium
DOI：
10.1073/pnas.1221442110
发表时间：
2013-03-26
影响因子：
11.1
作者：
Lee, Sung Chang;Bennett, Brad C.;Zhang, Qinghai
通讯作者：
Zhang, Qinghai
影响因子：
2.9
作者：
Lee, Sangbae;Ghosh, Soumadwip;Vaidehi, Nagarajan
通讯作者：
Vaidehi, Nagarajan
影响因子：
3.4
作者：
Abel, Stephane;Marchi, Massimo;Bonnete, Francoise
通讯作者：
Bonnete, Francoise
影响因子：
4.6
作者：
Chaptal V;Delolme F;Kilburg A;Magnard S;Montigny C;Picard M;Prier C;Monticelli L;Bornert O;Agez M;Ravaud S;Orelle C;Wagner R;Jawhari A;Broutin I;Pebay-Peyroula E;Jault JM;Kaback HR;le Maire M;Falson P
通讯作者：
Falson P