Comparison of serum and liver hepatitis C virus quasispecies in HCV-related hepatocellular carcinoma
Comparison of serum and liver hepatitis C virus quasispecies in HCV-related hepatocellular carcinoma
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DOI：
10.1016/s0168-8278(98)80115-3
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发表时间：
1998-12-01
影响因子：
25.7
通讯作者：
Pisi, E
中科院分区：
文献类型：
--
作者：
De Mitri, MS;Mele, L;Pisi, E
背景/目的：丙型肝炎病毒（HCV）基因组由异质变异的准种群体组成，特别是在高变区。为了评估丙型肝炎相关肝细胞癌的病毒准种概况，我们研究了13例丙型肝炎阳性的肝硬化和非肝硬化肝细胞癌患者（分别为5例和8例）的血清和肝组织中的病毒种群模式。方法：采用聚合酶链反应介导的单链构象多态性分析方法分析HCV基因组异质性，发现多个DNA条带代表不同的高变区序列。结果:在3/5合并肝硬化的肝细胞癌和6/8无肝硬化的肝细胞癌中，HCV种群在肿瘤组织和非肿瘤组织之间存在差异，除一例外，在所有肝细胞癌的两个区室中都检测到至少一个或多个共同带，在合并或不合并肝硬化的肝细胞癌中，HCV准种的复杂性没有显著差异。血清和肝组织HCV准种谱的比较显示，6/7例患者的HCV变异在这两个区室之间分布不同。在四个病例中，检测到共同序列和区隔序列，而在两个没有肝硬化的病例中，血清中的HCV种群与肝脏中的HCV种群完全不同。结论：这些结果表明，HCV群体的复杂性受肝细胞癌的存在而不是潜在慢性肝病的严重程度的影响，血清和肝脏中发现的不同准种模式可能反映了循环和肝内HCV颗粒的不同生物学特性或肝外复制位点的存在。
Background/Aims: The hepatitis C virus (HCV) genome consists of quasispecies populations of heterogeneous variants, especially in the hypervariable region, To assess the profiles of viral quasispecies in HCV-related hepatocellular carcinoma, we studied the viral population patterns in serum and liver tissues of 13 HCV-positive patients with hepatocellular carcinoma developed on cirrhotic and non-cirrhotic livers (5 and 8 cases, respectively).Methods: HCV genome heterogeneity was analyzed by polymerase chain reaction-mediated single-strand conformation polymorphism analysis, which showed multiple DNA bands representing different hypervariable region sequences.Results: The HCV populations were different between tumorous and nontumorous tissues in 3/5 hepatocellular carcinomas with cirrhosis and in 6/8 without cirrhosis, At least one or more than one common band was detected in both compartments in all but one case, No significant differences in the complexity of HCV quasispecies were found in hepatocellular carcinoma with or without underlying cirrhosis, Comparison of the HCV quasispecies profiles in serum and liver tissues showed a different distribution of HCV variants between these two compartments in 6/7 patients. In four cases, both common and compartmentalized sequences were detected, whereas in two cases, both without cirrhosis, the HCV population in serum was completely different from that found in the liver.Conclusions: These results suggest that the complexity of HCV populations is influenced by the presence of hepatocellular carcinoma rather than by the severity of the underlying chronic liver disease, The different quasispecies patterns found in serum and liver may reflect different biological properties of circulating and intrahepatic HCV particles or the existence of extrahepatic sites of replication.