Reduced activity of Arabidopsis chromosome-cohesion regulator gene CTF7/ECO1 alters cytosine methylation status and retrotransposon expression
Reduced activity of Arabidopsis chromosome-cohesion regulator gene CTF7/ECO1 alters cytosine methylation status and retrotransposon expression
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DOI：
10.1080/15592324.2015.1013794
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发表时间：
2015-05-04
影响因子：
2.9
通讯作者：
Jauh, Guang-Yuh
中科院分区：
文献类型：
--
作者：
Bolanos-Villegas, Pablo;Jauh, Guang-Yuh
多细胞生物，如高等植物，需要及时调节DNA复制和细胞分裂才能生长和发育。最近在拟南芥中的工作表明，减数分裂和有丝分裂过程中的染色体分离取决于几个基因的活性，这些基因在酵母中参与了染色体凝聚力的建立。在这个过程中，染色体结构维持蛋白(SMC)家族将染色体连接在一起，并建立染色体间和染色体内的连接。在拟南芥中，SMC蛋白的募集和细胞周期关键阶段的凝聚力的建立取决于染色体传递保真度7/凝聚力1(CTF7/ECO1)的活性。在这里，我们表明，CTF7/ECO1活性的丧失改变了基因间隔区和转座子基因座上胞嘧啶甲基化的状态。还观察到转座子Copia28的表达增加，这表明CTF7/ECO1活性、DNA甲基化和基因沉默之间存在联系。需要更多的工作来确定干预这一过程的机制关系。
Multicellular organisms such as higher plants require timely regulation of DNA replication and cell division to grow and develop. Recent work in Arabidopsis has shown that chromosome segregation during meiosis and mitosis depends on the activity of several genes that in yeast are involved in the establishment of chromosomal cohesion. In this process, proteins of the STRUCTURAL MAINTENANCE OF CHROMOSOMES (SMC) family tether chromosomes and establish inter- and intrachromosomal connections. In Arabidopsis, recruitment of SMC proteins and establishment of cohesion during key stages of the cell cycle depend on the activity of CHROMOSOME TRANSMISSION FIDELITY 7/ESTABLISHMENT OF COHESION 1 (CTF7/ECO1). Here we show that loss of CTF7/ECO1 activity alters the status of cytosine methylation in both intergenic regions and transposon loci. An increase in expression was also observed for transposon copia28, which suggests a link between CTF7/ECO1 activity, DNA methylation and gene silencing. More work is needed to determine the mechanistic relationships that intervene in this process.