Natural Variation in Arabidopsis Cvi-0 Accession Reveals an Important Role of MPK12 in Guard Cell CO2 Signaling.
Natural Variation in Arabidopsis Cvi-0 Accession Reveals an Important Role of MPK12 in Guard Cell CO2 Signaling.
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拟南芥 Cvi-0 的自然变异揭示了 MPK12 在保卫细胞 CO2 信号传导中的重要作用。
DOI：
10.1371/journal.pbio.2000322
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发表时间：
2016-12
期刊：
影响因子：
9.8
通讯作者：
Brosché M
中科院分区：
文献类型：
--
作者：
Jakobson L;Vaahtera L;Tõldsepp K;Nuhkat M;Wang C;Wang YS;Hõrak H;Valk E;Pechter P;Sindarovska Y;Tang J;Xiao C;Xu Y;Gerst Talas U;García-Sosa AT;Kangasjärvi S;Maran U;Remm M;Roelfsema MR;Hu H;Kangasjärvi J;Loog M;Schroeder JI;Kollist H;Brosché M
植物的气体交换是由保卫细胞调节的，保卫细胞形成气孔。气孔调节对植物生存至关重要;它们调节光合作用所需的CO2吸收、水分损失和空气污染物（如臭氧）的进入。我们映射臭氧敏感性，更开放的气孔，气孔CO2不敏感表型的拟南芥登录Cvi-0有丝分裂原激活蛋白（MAP）激酶12（MPK 12）的一个单一的氨基酸取代。同时，我们发现，气孔CO2不敏感表型的突变顺式（CO2不敏感）是由MPK 12的缺失。缺乏MPK 12损害碳酸氢盐诱导的S型阴离子通道的激活。我们证明了MPK 12与蛋白激酶高叶温度1（HT 1）相互作用-保卫细胞CO2信号传导的中心节点-MPK 12作为HT 1的抑制剂发挥作用。这些数据为植物MPKs作为蛋白激酶抑制剂提供了一种新的功能，并提出了一种机制，通过该机制，保卫细胞CO2信号控制植物水分管理。人类活动增加了对流层中二氧化碳和臭氧等有害空气污染物的浓度。这些变化可能对农业生产力产生不利影响。保卫细胞在叶片上形成气孔，调节植物的气体交换。为了维持光合作用，气孔打开以允许CO2吸收，但同时，开放的气孔导致水分流失并允许臭氧进入。大气CO2水平升高会降低气孔开度，这可以改善植物水分平衡，但也会增加叶片温度。利用遗传学方法，我们利用自然变异和突变体分析的塔勒水芹（拟南芥），我们发现，有丝分裂原激活蛋白激酶12（MPK 12）和它的抑制相互作用与另一个激酶，高叶温度1（HT 1）（参与保卫细胞CO2信号），在这一调控过程中发挥了关键作用。因此，我们已经确定了一种机制，其中保卫细胞CO2信号调节植物如何有效地利用水和科普空气污染物臭氧。
Plant gas exchange is regulated by guard cells that form stomatal pores. Stomatal adjustments are crucial for plant survival; they regulate uptake of CO2 for photosynthesis, loss of water, and entrance of air pollutants such as ozone. We mapped ozone hypersensitivity, more open stomata, and stomatal CO2-insensitivity phenotypes of the Arabidopsis thaliana accession Cvi-0 to a single amino acid substitution in MITOGEN-ACTIVATED PROTEIN (MAP) KINASE 12 (MPK12). In parallel, we showed that stomatal CO2-insensitivity phenotypes of a mutant cis (CO2-insensitive) were caused by a deletion of MPK12. Lack of MPK12 impaired bicarbonate-induced activation of S-type anion channels. We demonstrated that MPK12 interacted with the protein kinase HIGH LEAF TEMPERATURE 1 (HT1)—a central node in guard cell CO2 signaling—and that MPK12 functions as an inhibitor of HT1. These data provide a new function for plant MPKs as protein kinase inhibitors and suggest a mechanism through which guard cell CO2 signaling controls plant water management. Human activities have increased the concentrations of CO2 and harmful air pollutants such as ozone in the troposphere. These changes can have detrimental consequences for agricultural productivity. Guard cells, which form stomatal pores on leaves, regulate plant gas exchange. To maintain photosynthesis, stomata open to allow CO2 uptake, but at the same time, open stomata lead to loss of water and allow the entrance of ozone. Elevated atmospheric CO2 levels reduce stomatal apertures, which can improve plant water balance but also increases leaf temperature. Using genetic approaches—in which we exploit natural variation and mutant analysis of thale cress (Arabidopsis thaliana)—we find that MITOGEN-ACTIVATED PROTEIN KINASE 12 (MPK12) and its inhibitory interaction with another kinase, HIGH LEAF TEMPERATURE 1 (HT1) (involved in guard cell CO2 signaling), play a key role in this regulatory process. We have therefore identified a mechanism in which guard cell CO2 signaling regulates how efficiently plants use water and cope with the air pollutant ozone.
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影响因子：
64.8
作者：
Engineer, Cawas B.;Ghassemian, Majid;Anderson, Jeffrey C.;Peck, Scott C.;Hu, Honghong;Schroeder, Julian I.
通讯作者：
Schroeder, Julian I.
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1073/pnas.0907205106
发表时间：
2009-12-01
影响因子：
11.1
作者：
Jammes, Fabien;Song, Charlotte;Kwak, June M.
通讯作者：
Kwak, June M.
影响因子：
4.5
作者：
Liu J;Yang H;Bao F;Ao K;Zhang X;Zhang Y;Yang S
通讯作者：
Yang S
影响因子：
64.8
作者：
Keenan, Trevor F.;Hollinger, David Y.;Richardson, Andrew D.
通讯作者：
Richardson, Andrew D.