Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6
Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6
复制标题
DOI：
10.1111/j.1469-7793.2000.00237.x
复制
发表时间：
2000-11-15
影响因子：
5.5
通讯作者：
Pedersen, BK
中科院分区：
文献类型：
--
作者：
Steensberg, A;van Hall, G;Pedersen, BK
1.血浆白细胞介素（IL）-6浓度随着运动而增加，并且已经证明收缩的肌肉可以产生IL-6。在本研究中解决的问题是，通过收缩骨骼肌产生的IL-6是否具有如此大的量，以至于它可以解释IL-6在血液中的积累。这是在六个健康的男性，谁进行单腿动态膝伸肌运动5小时，在25 W，这代表了40%的峰值功率输出（W-max）。在运动前和运动期间每小时获得运动和休息腿的动脉-股静脉（a-fv）差异。通过超声多普勒技术平行测量腿部血流。采用酶联免疫吸附法（ELISA）测定IL-6.与休息相比，IL-6的动脉血浆浓度增加了19倍。运动腿上IL-6的a-fv差异遵循与净IL-B释放相同的模式。在静止腿上，没有显著的a-fv差异或净IL-6释放。这项工作是由2.5公斤的活动肌肉产生的，这意味着在最后2小时的运动中，IL-6的产生中位数为6.8 ng min（-1）（kg活动肌肉）（-1）（范围，3.96-9.69 ng min（-1）kg（-1））。在运动的最后2小时从肌肉中释放的净IL-6是动脉IL-6浓度升高的17倍，并且在运动的5小时，在1分钟期间的净释放是血浆中IL-6含量的一半。这表明在肌肉锻炼期间IL-6的转换非常高。我们认为，骨骼肌产生的IL-6有助于维持葡萄糖稳态在长时间的运动。
1. Plasma interleukin (IL)-6 concentration is increased with exercise and it has been demonstrated that contracting muscles can produce IL-6. The question addressed in the present study was whether the IL-6 production by contracting skeletal muscle is of such a magnitude that it can account for the IL-6 accumulating in the blood.2. This was studied in six healthy males, who performed one-legged dynamic knee extensor exercise for 5 h at 25 W, which represented 40% of peak power output (W-max). Arterial-femoral venous (a-fv) differences over the exercising and the resting leg were obtained before and every hour during the exercise. Leg blood flow was measured in parallel by the ultrasound Doppler technique. IL-6 was measured by enzyme-linked immunosorbent assay (ELISA).3. Arterial plasma concentrations for IL-6 increased 19-fold compared to rest. The a-fv difference for IL-6 over the exercising leg followed the same pattern as did the net IL-B release. Over the resting leg, there was no significant a-fv difference or net IL-6 release. The work was produced by 2.5 kg of active muscle, which means that during the last 2 h of exercise, the median IL-6 production was 6.8 ng min(-1) (kg active muscle)(-1) (range, 3.96-9.69 ng min(-1) kg(-1)).4. The net IL-6 release from the muscle over the last 2 h of exercise was 17-fold higher than the elevation in arterial IL-6 concentration and at 5 h of exercise the net release during 1 min was half of the IL-6 content in the plasma. This indicates a very high turnover of IL-6 during muscular exercise. We suggest that IL-6 produced by skeletal contracting muscle contributes to the maintenance of glucose homeostasis during prolonged exercise.