Effects of plant species on stream bacterial communities via leachate from leaf litter
Effects of plant species on stream bacterial communities via leachate from leaf litter
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DOI：
10.1007/s10750-017-3386-x
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发表时间：
2018-02
期刊：
影响因子：
2.6
通讯作者：
A. Wymore;Elena Salpas;G. Casaburi;Cindy M. Liu;L. Price;B. Hungate;W. McDowell;J. Marks
中科院分区：
文献类型：
--
作者：
A. Wymore;Elena Salpas;G. Casaburi;Cindy M. Liu;L. Price;B. Hungate;W. McDowell;J. Marks
凋落叶是森林河流生态系统的重要资源。在落叶过程中，大量的溶解有机碳（DOC）进入河流的树叶渗滤液。我们比较了植物种类和叶渗滤液的生物有效性的流细菌群落组成和DOC分解率的影响。我们使用了四种常见的河岸树种，不同的叶化学，渗滤液的光学性质，和凋落物分解率。我们使用的实验室缩影从两个流和修正与标准浓度的DOC来自叶沥滤液的四个树种。24小时后，我们测量DOC的生物降解速率，并通过16S rRNA基因的条形码焦磷酸测序确定细菌群落的组成。的组成，多样性和丰富的细菌群落之间的植物物种从两个流显着不同。不同细菌群落的系统发育距离与物种特异性渗滤液的光学性质和DOC生物降解速率相关。DOC分解率最高与高单宁和木质素叶型。结果表明，河岸植物物种通过其渗滤液强烈影响溪流细菌群落，这表明河岸植物分类群的存在或丰度的改变可能会影响这些群落和相关的生态系统过程。
Leaf litter provides an important resource to forested stream ecosystems. During leaf fall a significant amount of dissolved organic carbon (DOC) enters streams as leaf leachate. We compared the effects of plant species and leaf leachate bioavailability on the composition of stream bacterial communities and rates of DOC decomposition. We used four common riparian tree species that varied in foliar chemistry, leachate optical properties, and litter decomposition rate. We used laboratory microcosms from two streams and amended with a standard concentration of DOC derived from leaf leachate of the four tree species. After 24 h, we measured rates of DOC biodegradation and determined the composition of the bacterial communities via bar-coded pyrosequencing of the 16S rRNA gene. The composition, diversity, and abundance of the bacterial community differed significantly among plant species from both streams. The phylogenetic distance of the different bacterial communities correlated with species-specific leachate optical properties and rates of DOC biodegradation. Highest rates of DOC decomposition were associated with high tannin and lignin leaf types. Results demonstrate that riparian plant species strongly influences stream bacterial communities via their leachate suggesting that alterations to the presence or abundance of riparian plant taxa may influence these communities and associated ecosystem processes.