Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro
Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro
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DOI：
10.1016/j.biomaterials.2003.08.045
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发表时间：
2004-03-01
期刊：
影响因子：
14
通讯作者：
Park, WH
中科院分区：
文献类型：
--
作者：
Min, BM;Lee, G;Park, WH
采用静电纺丝法制备了用于正常人角质形成细胞和成纤维细胞培养的丝素纤维非织造布。以甲酸为纺丝溶剂对再生丝素纤维进行静电纺丝。为了不溶解，用50%的甲醇水溶液对刚纺成的SF无纺布进行化学处理。采用扫描电镜和压汞法研究了初生丝素纤维和化学处理丝素纤维的形貌和微观结构。初纺SF纳米纤维具有光滑表面的圆形横截面。从图像分析，它们的平均直径为80 nm，它们的直径范围为30 - 120 nm。在化学处理60 min期间，由SF纳米纤维组成的非织造布的孔隙率从76.1%下降到68.1%。为了分析静电纺丝SF纳米纤维上的细胞相容性和细胞行为，研究了正常人角质形成细胞和成纤维细胞在SF纳米纤维上的附着和铺展以及细胞与SF纳米纤维之间的相互作用。通过扫描电子显微镜检查SF纳米纤维上的细胞形态。我们的研究结果表明，SF纳米纤维可能是一个很好的候选人的生物医学应用，如伤口敷料和组织工程支架。(C)2003 Elsevier Ltd.保留所有权利。
An electrospinning method was used to fabricate silk fibroin (SF) nanofiber nonwovens for cell culture of normal human keratinocytes and fibroblasts. The electrospinning of regenerated SF was performed with formic acid as a spinning solvent. For insolubilization, as-spun SF nanofiber nonwovens were chemically treated with an aqueous methanol solution of 50%. Morphology and microstructure of as-spun and chemically treated SF nanofibers were investigated by scanning electron microscopy and mercury porosimetry. As-spun SF nanofibers exhibited a circular cross-section with a smooth surface. From the image analysis, they had an average diameter of 80nm and their diameters ranged from 30 to 120nm. During the chemical treatment for 60 min, porosity of nonwovens composed of SF nanofibers decreased from 76.1% up to 68.1%. To assay the cytocompatibility and cell behavior onto the electrospun SF nanofibers, cell attachment and spreading of normal human keratinocytes and fibroblasts seeded on the SF nanofibers and interaction between cells and SF nanofibers were studied. Cell morphology on SF nanofibers was examined by scanning electron microscopy. Our results indicate that the SF nanofibers may be a good candidate for the biomedical applications, such as wound dressing and scaffolds for tissue engineering. (C) 2003 Elsevier Ltd. All rights reserved.