Early acquisition of Pneumocystis carinii in neonatal rats using targeted PCR and oral swabs.
Early acquisition of Pneumocystis carinii in neonatal rats using targeted PCR and oral swabs.
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使用靶向 PCR 和口腔拭子在新生大鼠中早期采集卡氏肺孢子虫。
DOI：
10.1111/j.1550-7408.2001.tb00486.x
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Cushion,MT
中科院分区：
文献类型：
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作者：
Icenhour,CR;Rebholz,SL;Collins,MS;Cushion,MT
选取10只大鼠及其幼鼠（121只大鼠）。繁殖大鼠和新生大鼠与其他已知的窝藏大鼠关在一起。囊虫囊虫(Pcc)。在1-4周龄时，采用口腔拭子/聚合酶链反应技术对幼鼠进行取样（1周龄8只，2周龄13只，4周龄11只）。所有31份样本及其样本（n=4）均为pcc特异性DNA阳性。在出生后1-2小时、24小时和1周分别从3只大鼠坝及其幼崽中采集第二组口天鹅。在出生后的2小时内，78%（36只中的28只）的幼崽的pc特异性DNA呈阳性。24 h和1周龄时，100%的pcc特异性DNA阳性。在所有样本时间点，3个水坝的pc特异性DNA也呈阳性。于3个时间点（妊娠2 ~ 4周）剖宫产大鼠仔。48只大鼠胎儿、胎盘或蜕膜均未检测出pcc特异性DNA阳性。口腔拭子、肺组织匀浆和脾脏匀浆均为阳性。
10 rat dams and their pups were included in the study (121 rats). Breeder rats and newborn rats were housed with other rats known to harbourP. carinii carinii(Pcc). Rat pups were sampled by the oral swab/polymerase chain reaction technique at ages 1-4 weeks (8 pups at 1 week, 13 pups at 2 weeks and 11 pups at 4 weeks). All 31 samples and their dams (n=4) were positive for Pcc-specific DNA by. A second set of oral swans were collected from 3 rat dams and their pups at 1-2 h, 24 h and 1 week after birth. 78% (28 of 36) of the pups were positive for Pcc-specific DNA within the first 2 h of life. By 24 h and at 1 week of age, 100% were positive for Pcc-specific DNA. The 3 dams were also positive for Pcc-specific DNA during all sample time points. Rat pups were collected by caesarean section at 3 time points (2-4 weeks of gestation). None of the 48 rat fetuses, placentas or deciduae were positive for Pcc-specific DNA. The 3 dams were positive by oral swab, lung tissue homogenate and spleen homogenate.